| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RYBP |
| Uniprot No | Q8N488 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-228aa |
| 氨基酸序列 | MTMGDKKSPT RPKRQAKPAA DEGFWDCSVC TFRNSAEAFK CSICDVRKGT STRKPRINSQ LVAQQVAQQY ATPPPPKKEK KEKVEKQDKE KPEKDKEISP SVTKKNTNKK TKPKSDILKD PPSEANSIQS ANATTKTSET NHTSRPRLKN VDRSTAQQLA VTVGNVTVII TDFKEKTRSS STSSSTVTSS AGSEQQNQSS SGSESTDKGS SRSSTPKGDM SAVNDESF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RYBP重组蛋白的3-4篇参考文献示例(文献信息为模拟概括，仅供参考)：
1. **"RYBP interacts with PRC1 components and regulates target genes through H2A ubiquitination"**
- 作者：Wang et al.
- 摘要：研究揭示了RYBP重组蛋白作为PRC1复合体的关键成员，通过调控组蛋白H2A的泛素化修饰影响基因沉默，尤其在胚胎干细胞分化中起重要作用。
2. **"RYBP is essential for murine embryogenesis and modulates Polycomb-mediated gene repression"**
- 作者：Gonzalez et al.
- 摘要：通过小鼠模型证明RYBP缺失导致胚胎致死，其通过增强Polycomb复合体的抑制功能参与发育相关基因的表观遗传调控。
3. **"RYBP suppresses tumorigenesis by stabilizing p53 and inhibiting DNA damage response"**
- 作者：Hisada et al.
- 摘要：发现RYBP重组蛋白通过结合并稳定p53蛋白抑制肿瘤生长，并揭示其在DNA损伤修复通路中的拮抗作用。
4. **"Structural basis of RYBP function in chromatin remodeling and apoptosis"**
- 作者：Zhang et al.
- 摘要：利用重组RYBP蛋白解析其晶体结构，阐明其通过结合特定染色质区域诱导细胞凋亡的分子机制。
(注：以上文献标题和作者为学术场景模拟，实际引用需以真实发表的论文为准。)
**Background of RYBP Recombinant Protein**
RYBP (RING1 and YY1 Binding Protein) is a multifunctional regulatory protein involved in epigenetic modulation, transcriptional control, and cellular processes such as apoptosis and differentiation. It was initially identified as an interacting partner of the Polycomb group (PcG) proteins, specifically RING1 and YY1. which are core components of the Polycomb repressive complex 1 (PRC1). PRC1 plays a critical role in gene silencing by catalyzing histone H2A ubiquitination, a key step in chromatin remodeling and maintenance of transcriptional repression during development and cell fate determination.
RYBP also interacts with non-Polycomb proteins, linking it to pathways like DNA damage response and tumor suppression. Structurally, RYBP contains a ubiquitin-binding domain and a conserved N-terminal region, enabling its participation in diverse protein complexes. Studies highlight its dual roles in cancer: it can act as a tumor suppressor by promoting apoptosis or as an oncogenic factor by enhancing cell proliferation, depending on cellular context.
Recombinant RYBP protein is engineered using expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, biologically active forms for *in vitro* studies. This tool enables researchers to investigate RYBP’s molecular mechanisms—such as its interplay with PRC1 in epigenetic regulation, its role in embryonic development, or its impact on cancer pathways. Applications include protein interaction assays, enzymatic activity analyses, and drug discovery targeting RYBP-associated diseases. Its recombinant form is critical for unraveling the complexity of epigenetic networks and developing therapeutic strategies for cancers, neurological disorders, and developmental abnormalities linked to PcG dysfunction.
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