| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RWDD1 |
| Uniprot No | Q9H446 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-243aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMTDYGEEQRNELEALESIYPDSFTVLS ENPPSFTITVTSEAGENDETVQTTLKFTYSEKYPDEAPLYEIFSQENLED NDVSDILKLLALQAEENLGMVMIFTLVTAVQEKLNEIVDQIKTRREEEKK QKEKEAEEAEKQLFHGTPVTIENFLNWKAKFDAELLEIKKKRMKEEEQAG KNKLSGKQLFETDHNLDTSDIQFLEDAGNNVEVDESLFQEMDDLELEDDE DDPDYNPADPESDSAD |
| 预测分子量 | 30 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RWDD1重组蛋白的参考文献示例(注：由于RWDD1研究相对较少，部分文献为假设性示例，建议通过PubMed或专业数据库核实最新研究)：
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1. **文献名称**: *"Cloning, expression, and functional characterization of recombinant RWDD1 in ubiquitination pathways"*
**作者**: Li X, et al.
**摘要**: 本研究通过构建RWDD1重组蛋白表达载体，在大肠杆菌中实现高效可溶性表达，并验证其通过RWD结构域参与泛素连接酶复合物的组装，调控底物蛋白降解过程。
2. **文献名称**: *"Structural insights into the interaction of RWDD1 with UBC12 in neddylation"*
**作者**: Zhang Y, et al.
**摘要**: 利用重组RWDD1蛋白进行X射线晶体学分析，揭示其与UBC12的结合模式，证明RWDD1作为辅助因子在蛋白质neddylation修饰中的调控作用。
3. **文献名称**: *"RWDD1 promotes cell proliferation via MAPK signaling: Evidence from recombinant protein-based assays"*
**作者**: Wang H, et al.
**摘要**: 通过体外重组RWDD1蛋白处理细胞实验，发现其通过激活ERK/MAPK通路增强肿瘤细胞增殖能力，提示其作为癌症治疗潜在靶点。
4. **文献名称**: *"Development of a high-throughput screening assay for RWDD1 interactors using recombinant protein pull-down"*
**作者**: Kim S, et al.
**摘要**: 建立基于重组RWDD1蛋白的Pull-down联合质谱技术平台，筛选出多个新型互作蛋白，拓展了对RWDD1在DNA损伤应答中功能的认识。
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**注意**：以上文献为模拟示例，实际研究中RWDD1相关重组蛋白的报道较少，建议通过**NCBI PubMed**或**Web of Science**以“RWDD1 recombinant protein”为关键词检索最新成果。
RWDD1 (RWD Domain-Containing Protein 1) is a relatively understudied protein encoded by the *RWDD1* gene located on human chromosome 9q34.3. It contains a conserved RWD domain, a structural motif implicated in protein-protein interactions, and a C-terminal WD40 repeat domain, which often participates in signal transduction and scaffolding. While its precise biological functions remain unclear, RWDD1 has been linked to cellular processes such as transcriptional regulation, apoptosis, and DNA damage response. Studies suggest it may interact with ubiquitination machinery or modulate stress-responsive pathways, though mechanistic details are sparse.
Recombinant RWDD1 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its production faces challenges due to potential post-translational modifications and structural complexity. Purified recombinant RWDD1 serves as a critical tool for investigating its binding partners, enzymatic activities, and subcellular localization. Research applications include in vitro binding assays, structural analysis (e.g., crystallography), and antibody generation. Notably, RWDD1 has been associated with cancer progression and neurological disorders in preliminary studies, sparking interest in its potential as a biomarker or therapeutic target. However, validation of these roles requires deeper exploration of its molecular interactions and tissue-specific expression patterns. Current efforts focus on deciphering its role in cellular stress adaptation and disease pathogenesis using recombinant protein-based approaches.
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