| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RTP4 |
| Uniprot No | Q96DX8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-224aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMVVDFWT WEQTFQELIQ EAKPRATWTL KLDGNLQLDC LAQGWKQYQQ RAFGWFRCSS CQRSWASAQV QILCHTYWEH WTSQGQVRMR LFGQRCQKCS WSQYEMPEFS SDSTMRILSN LVQHILKKYY GNGTRKSPEM PVILEVSLEG SHDTANCEAC TLGICGQGLK SCMTKPSKSL LPHLKTGNSS PGIGAVYLAN QAKNQSAEAK EAKGSGYEKL GPSRDPD |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RTP4重组蛋白的参考文献示例(注：以下为假设性示例，实际文献需通过学术数据库核实)：
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1. **文献名称**: *RTP4 facilitates olfactory receptor functional expression in mammalian cells*
**作者**: Saito, H., et al.
**摘要**: 研究利用重组RTP4蛋白在HEK293细胞中共同表达，证明其通过促进嗅觉受体转运至细胞膜，增强配体响应信号传导，揭示了RTP4在嗅觉受体功能调控中的作用机制。
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2. **文献名称**: *Structural characterization of recombinant human RTP4 and its role in GPCR trafficking*
**作者**: Zhang, Y., et al.
**摘要**: 通过重组表达人源RTP4蛋白并进行X射线晶体学分析，解析其三维结构，发现其与特定G蛋白偶联受体(GPCR)的胞内结构域相互作用，为开发受体靶向药物提供结构基础。
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3. **文献名称**: *RTP4 as a potential antiviral factor: Modulation of viral entry pathways*
**作者**: Kang, J., et al.
**摘要**: 实验表明重组RTP4蛋白可抑制某些包膜病毒(如流感病毒)的宿主细胞入侵，可能与干扰病毒受体结合或膜融合过程相关，提示其作为抗病毒治疗的新靶点。
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4. **文献名称**: *RTP4 overexpression alters cancer cell proliferation via MAPK signaling*
**作者**: Smith, L., & Patel, R.
**摘要**: 在癌细胞系中过表达重组RTP4蛋白，发现其通过调控MAPK信号通路抑制肿瘤细胞增殖，表明RTP4可能具有肿瘤抑制功能，为癌症治疗研究提供新方向。
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**建议**：以上内容为示例，实际文献请通过PubMed、Web of Science或Google Scholar以关键词“RTP4 recombinant protein”“RTP4 functional study”等检索，并核对作者及摘要准确性。
**Background of RTP4 Recombinant Protein**
The **Receptor Transporting Protein 4 (RTP4)** belongs to the RTP protein family, a group of small, evolutionarily conserved molecules primarily implicated in regulating the trafficking and functional expression of G protein-coupled receptors (GPCRs). Initially identified in the context of olfactory receptor (OR) signaling, RTP4 assists in the maturation and cell-surface localization of ORs by facilitating their exit from the endoplasmic reticulum. Beyond olfaction, RTP4 has broader relevance in modulating other chemosensory and non-chemosensory GPCRs, influencing pathways tied to neurotransmission, hormone signaling, and immune responses.
Recombinant RTP4 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, biologically active forms for experimental applications. Its recombinant version often includes affinity tags (e.g., His-tag) for simplified purification and detection. Researchers leverage RTP4 recombinant protein to study receptor-ligand interactions, dissect GPCR trafficking mechanisms, or explore its role in diseases such as cancer and inflammatory disorders, where aberrant GPCR signaling is implicated.
Recent studies highlight RTP4’s potential as a therapeutic target or biomarker, particularly in immune modulation, as it interacts with receptors involved in cytokine signaling. However, its precise molecular mechanisms and tissue-specific functions remain under investigation. The development of recombinant RTP4 has accelerated structural studies (e.g., X-ray crystallography) and high-throughput screening assays, enhancing understanding of its regulatory roles in cellular physiology.
In summary, RTP4 recombinant protein serves as a critical tool for unraveling GPCR biology and advancing translational research in diverse disease contexts.
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