关键信息
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基因名
AMPK gamma 1/beta 1/alpha 1 Heterotrimer
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简介
AMPK gamma 1 is the AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK) and is an important regulator of cellular energy metabolism. It is activated by low ATP levels, enhancing energy production while inhibiting biosynthetic processes and cell growth. AMPK gamma 1/beta 2/alpha 1 Heterotrimer Protein, Human (sf9, His-GST) is the recombinant human-derived AMPK gamma 1/beta 2/alpha 1 Heterotrimer protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. AMPK gamma 1/beta 2/alpha 1 Heterotrimer Protein, Human (sf9, His-GST), has molecular weight of ~35 & 37 & 95 KDa, respectively.
- 应用
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别名
5'-AMP-activated protein kinase; AMPK; AMPK (α1, β2, γ1) Protein
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种属
Human
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表达系统
Baculovirus
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标签
N-His;N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P54619 (M1-P331)&O43741 (M1-I272)&Q13131
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表达区间
P54619 (M1-P331)&O43741 (M1-I272)&Q13131 (M1-Q559)
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分子量
35&37&95 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
AMP-activated protein kinase (AMPK) is a vital energy sensor in cellular metabolism, composed of three subunits: alpha (α), beta (β), and gamma (γ). The heterotrimeric complex, specifically the AMPK gamma 1 (γ1), beta 1 (β1), and alpha 1 (α1) subunits, plays a crucial role in regulating energy homeostasis by activating catabolic pathways and inhibiting anabolic processes in response to cellular energy depletion. The research into AMPK's structure and function has intensified due to its implications in metabolic diseases, such as obesity, diabetes, and cancer. The ability to produce recombinant AMPK heterotrimers in vitro has provided valuable insights into the intricate mechanisms of AMPK signaling and its activation by AMP and other nucleotides. Understanding the specific interactions and regulatory mechanisms of the AMPK γ1/β1/α1 complex enhances our knowledge of metabolic regulation and may lead to novel therapeutic strategies targeting this essential kinase to combat metabolic disorders and improve overall health. Thus, studying the recombinant protein forms of AMPK subunits serves as a fundamental approach to elucidating their functional roles within the signaling pathways that govern energy metabolism.
