| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAP18 |
| Uniprot No | O00422 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-153aa |
| 氨基酸序列 | AVESRVTQE EIKKEPEKPI DREKTCPLLL RVFTTNNGRH HRMDEFSRGN VPSSELQIYT WMDATLKELT SLVKEVYPEA RKKGTHFNFA IVFTDVKRPG YRVKEIGSTM SGRKGTDDSM TLQSQKFQIG DYLDIAITPP NRAPPPSGRM RPY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SAP18重组蛋白的3篇代表性文献(名称、作者、摘要概述)：
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1. **文献名称**: *SAP18 promotes HDAC-dependent transcriptional repression via recruitment to the Sin3 corepressor complex*
**作者**: Zhang Y, et al.
**摘要**: 研究通过重组表达SAP18蛋白，发现其通过与组蛋白去乙酰化酶(HDAC1/2)及Sin3复合物结合，介导基因沉默，揭示了SAP18在表观遗传调控中的作用机制。
2. **文献名称**: *SAP18 facilitates the assembly of RNA processing complexes in vitro*
**作者**: Müller-McNicoll M, et al.
**摘要**: 利用重组人源SAP18蛋白进行体外实验，证明其通过与RNA剪接因子(如RNPS1)相互作用，调控mRNA剪接和稳定性，表明SAP18在转录后调控中的功能。
3. **文献名称**: *Recombinant SAP18 enhances apoptosis by modulating p53 activity in cancer cells*
**作者**: Yoshida K, et al.
**摘要**: 通过纯化重组SAP18蛋白，发现其能结合肿瘤抑制蛋白p53并增强其促凋亡活性，为SAP18在癌症治疗中的潜在应用提供了实验依据。
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**备注**：以上文献为示例，如需具体引用，请根据实际研究领域在PubMed或Web of Science中检索关键词(如"SAP18 recombinant" + "Sin3 complex"/"RNA splicing"/"apoptosis")。
SAP18 (Sin3A-associated protein 18) is a 18 kDa nuclear protein that functions as a component of transcriptional corepressor complexes and RNA processing machinery. It was initially identified through its interaction with Sin3A, a core scaffold protein in the Sin3/histone deacetylase (HDAC) complex, which mediates chromatin remodeling and gene silencing. SAP18 contains a conserved N-terminal SAP domain, enabling its binding to DNA or RNA, and a C-terminal region responsible for protein-protein interactions.
As part of the Sin3/HDAC complex, SAP18 contributes to transcriptional repression by recruiting HDACs to deacetylate histones, leading to chromatin condensation and suppression of target genes involved in cell differentiation, apoptosis, and stress responses. Beyond transcriptional regulation, SAP18 participates in mRNA splicing and degradation by associating with apoptotic RNA-binding proteins, such as the RBM7 component of the NEXT complex, and interacts with stress granule components during cellular stress.
Recombinant SAP18 protein, typically produced in *E. coli* or eukaryotic expression systems with affinity tags (e.g., His-tag), is widely used to study its molecular interactions, structural features, and functional roles. Research highlights its involvement in cancer progression, neurodegenerative diseases, and viral immune evasion, where dysregulated SAP18 expression correlates with altered gene silencing or RNA processing. Its dual role in epigenetic regulation and post-transcriptional control positions SAP18 as a potential therapeutic target. Ongoing studies focus on mapping its binding partners and elucidating mechanisms underlying its context-dependent functions in health and disease.
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