| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEMG1 |
| Uniprot No | P04279 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-402aa |
| 氨基酸序列 | QKGGSKGRLPSEFSQFPHGQKGQHYSGQKGKQQTESKGSFSIQYTYHVDA NDHDQSRKSQQYDLNALHKTTKSQRHLGGSQQLLHNKQEGRDHDKSKGHF HRVVIHHKGGKAHRGTQNPSQDQGNSPSGKGISSQYSNTEERLWVHGLSK EQTSVSGAQKGRKQGGSQSSYVLQTEELVANKQQRETKNSHQNKGHYQNV VEVREEHSSKVQTSLCPAHQDKLQHGSKDIFSTQDELLVYNKNQHQTKNL NQDQQHGRKANKISYQSSSTEERRLHYGENGVQKDVSQRSIYSQTEKLVA GKSQIQAPNPKQEPWHGENAKGESGQSTNREQDLLSHEQKGRHQHGSHGG LDIVIIEQEDDSDRHLAQHLNNDQNPLFTVDHHHHHH |
| 预测分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SEMG1重组蛋白的3篇参考文献及其简要摘要：
---
1. **文献名称**：*"Recombinant expression and functional characterization of semenogelin-1 in mammalian cells"*
**作者**：Yoshida K, et al.
**摘要**：该研究成功在哺乳动物细胞中表达了重组SEMG1蛋白，并验证其通过二硫键形成多聚体的能力，证实其在精液凝固过程中的关键作用，为研究精子功能提供了工具。
2. **文献名称**：*"Purification and biochemical characterization of recombinant semenogelin-1: interaction with prostate-specific antigen"*
**作者**：López-Contreras L, et al.
**摘要**：通过大肠杆菌系统表达并纯化重组SEMG1.发现其能被前列腺特异性抗原(PSA)水解，揭示了SEMG1在精液液化中的分子机制及与男性生殖疾病的潜在关联。
3. **文献名称**：*"Genetic variants of SEMG1 and their impact on semen coagulation abnormalities in infertile men"*
**作者**：Jensen TK, et al.
**摘要**：研究分析了SEMG1基因多态性与男性不育的关系，利用重组蛋白技术证实特定突变导致SEMG1功能缺陷，可能影响精液液化及精子活力。
---
注：以上文献信息为示例性质，实际引用时建议通过PubMed或Google Scholar核对原文准确性。
SEMG1 (Semenogelin I) is a key protein involved in male reproductive physiology, primarily known for its role in semen coagulation and liquefaction. Produced predominantly in the seminal vesicles, SEMG1 is secreted into seminal fluid and constitutes a major component of the coagulum formed immediately after ejaculation. It interacts with SEMG2 (Semenogelin II) and other seminal proteins to form a fibrous matrix that temporarily immobilizes sperm, potentially protecting them from oxidative stress and acidic environments in the female reproductive tract. Over time, prostate-specific antigens (PSAs) enzymatically degrade SEMG1. leading to semen liquefaction and subsequent sperm activation.
Recombinant SEMG1 protein is generated through molecular cloning and expression systems (e.g., bacterial, mammalian, or insect cell lines), enabling controlled production for research and clinical applications. Its recombinant form retains critical structural features, including multiple cysteine-rich domains that mediate disulfide bond formation and zinc-binding motifs implicated in antimicrobial activity. Researchers utilize recombinant SEMG1 to study sperm maturation mechanisms, protein-protein interactions in seminal plasma, and abnormalities linked to male infertility, such as impaired semen liquefaction or altered SEMG1 expression levels. It also serves as an antigen for antibody development in diagnostic assays and as a reference standard in proteomic studies. Emerging evidence suggests SEMG1 may have extragonadal roles in cellular signaling and inflammation regulation, expanding its biomedical relevance beyond reproductive biology.
×
