首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠高迁移率族蛋白B1(HMGB-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的HMGB1含量。HMGB1 是一种广泛存在于真核细胞内的非组蛋白染色体结合蛋白。它参与多种生理和病理过程，如炎症反应调节等。其研究机制主要围绕其释放途径、与受体相互作用及下游信号传导通路，深入研究旨在更好理解疾病发生发展并寻找干预靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础医学研究中炎症反应机制探索、细胞损伤模型评估、免疫调控实验以及药物开发过程中对HMGB - 1表达水平的动态监测，其宽泛的样本兼容性为神经科学、肿瘤学、免疫学等领域的科研工作者提供了高效稳定的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hmgb1 ELISA Kit; Hmg-1 ELISA Kit; Hmg1High mobility group protein B1 ELISA Kit; High mobility group protein 1 ELISA Kit; HMG-1 ELISA Kit
缩写：HMGB1
Uniprot No.：P63158
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HMGB-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 92 | ||||
| Range % | 80-98 | |||||
| 1:200 | Average % | 85 | ||||
| Range % | 81-96 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 90-104 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of mouse HMGB-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 95-100 | ||||
| EDTA plasma (n=4) | 95 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7562.7122.7342.5655002.2232.2512.2372.0682501.5471.5721.5601.3911251.0200.9861.0030.83462.50.5980.6210.6100.44131.20.4520.4320.4420.27315.60.2870.2750.2810.11200.1730.1640.169 | ||||||
货期：3-5 working days
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