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产品描述：猪高迁移率族蛋白B1(HMGB1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HMGB1含量。HMGB1（高迁移率族蛋白B1）是一种在哺乳动物细胞中高度保守的核蛋白，参与DNA损伤修复、基因转录、炎症反应等生理和病理过程。其可通过细胞内外释放，作为损伤相关分子模式（DAMPs）信号，调节炎症、免疫、迁移等，并在多种疾病中发挥重要作用。研究HMGB1的机制有助于开发新的治疗策略。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于实验动物模型中的炎症性疾病研究、组织损伤修复机制探索，以及败血症、自身免疫疾病等病理过程中HMGB1的动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HMGB1 ELISA Kit; HMG1High mobility group protein B1 ELISA Kit; High mobility group protein 1 ELISA Kit; HMG-1 ELISA Kit
缩写：HMGB1
Uniprot No.：P12682
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig HMGB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:10 | Average % | 104 | ||||
| Range % | 100-108 | |||||
| 1:20 | Average % | 87 | ||||
| Range % | 84-91 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 90-96 | |||||
回收率：
| The recovery of pig HMGB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 93 | 87-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.1422.1652.1542.020101.6341.6451.6401.50651.0981.0571.0780.9442.50.6350.6010.6180.4841.250.4310.4120.4220.2880.6250.2980.2850.2920.1580.3120.2110.2150.2130.07900.1350.1330.134 | ||||||
货期：3-5 working days
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