首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血红素氧合酶1(HO-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的HMOX1含量。HMOX1 是一个关键靶点。它参与体内血红素代谢，具有抗氧化、抗炎等作用。研究发现其表达变化与多种疾病如心血管疾病、肿瘤等相关，机制涉及调节细胞应激反应、信号通路等，深入研究它对疾病防治有重要意义。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品可为研究HO-1在氧化应激相关疾病（如缺血再灌注损伤、神经退行性疾病）、炎症反应调控机制或代谢性疾病模型中的动态表达提供稳定工具，亦可用于评估药物或化合物对HO-1信号通路的影响，为生命科学基础研究与药物开发提供技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hmox1 ELISA Kit; Heme oxygenase 1 ELISA Kit; HO-1 ELISA Kit; EC 1.14.14.18 ELISA Kit; P32 protein ELISA Kit
缩写：HMOX1
Uniprot No.：P14901
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 92 | ||||
| Range % | 89-96 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 93-102 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of mouse HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 93-101 | ||||
| EDTA plasma (n=4) | 91 | 85-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.4162.3122.3642.17310001.8271.9061.8671.6765001.2471.2651.2561.0652500.7990.8290.8140.6231250.4770.4860.4820.29162.50.3340.3620.3480.15731.250.2570.2690.2630.07200.1940.1880.191 | ||||||
货期：3-5 working days
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