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产品描述：人羟甲基戊二酸单酰辅酶A还原酶 (HMG-CoAR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HMGCR含量。HMGCR即3-羟基-3-甲基戊二酰辅酶A还原酶，是胆固醇合成过程中的关键限速酶。其背景在于胆固醇代谢与多种疾病密切相关。研究机制主要聚焦于对其活性的调控，抑制该靶点可减少胆固醇合成，为降血脂药物研发提供了重要方向。试剂盒检测范围为0.78 ng/mL- 50 ng/mL，适用于基础医学研究中胆固醇代谢机制探索、他汀类药物作用效果评估、动脉粥样硬化模型建立等场景，亦可用于肝脏代谢相关疾病研究或药物开发过程中靶标蛋白的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：3 hydroxy 3 methylglutaryl CoA reductase ELISA Kit; 3 hydroxy 3 methylglutaryl Coenzyme A reductase ELISA Kit; 3 hydroxymethylglutaryl CoA reductase ELISA Kit; 3-hydroxy-3-methylglutaryl CoA reductase (NADPH) ELISA Kit; 3-hydroxy-3-methylglutaryl-coenzyme A reductase ELISA Kit; 3H3M ELISA Kit; HMDH_HUMAN ELISA Kit; HMG CoA reductase ELISA Kit; HMG CoAR ELISA Kit; HMG-CoA reductase ELISA Kit; Hmgcr ELISA Kit; Hydroxymethylglutaryl CoA reductase ELISA Kit; LDLCQ3 ELISA Kit; MGC103269 ELISA Kit; Red ELISA Kit
缩写：HMGCR
Uniprot No.：P04035
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL- 50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HMG-CoAR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 92-100 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 82-96 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 87-100 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of HMG-CoAR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-101 | ||||
| EDTA plasma (n=4) | 90 | 82-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.3252.4502.3882.271252.0502.1742.1121.99512.51.4761.5631.5201.4036.250.6590.6970.6780.5613.120.2450.2610.2530.1361.560.1920.1860.1890.0720.780.1510.1400.1460.02900.1200.1130.117 | ||||||
货期：3-5 working days
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