首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血红素氧合酶2(HO-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的HMOX2含量。HMOX2，全称血红素加氧酶2，是一种关键酶，催化血红素氧化裂解，释放一氧化碳和胆绿素，同时释放二价铁。研究显示，HMOX2基因在藏族高原低氧适应中发挥作用，调节血红蛋白代谢，实现低氧环境下的遗传适应。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品广泛用于基础医学研究，例如探索氧化应激相关疾病的分子机制、评估药物或基因干预对 HO - 2 表达的影响，或用于细胞模型中炎症及代谢调控通路的分析，为科研人员提供高效可靠的工具。适用于科研场景中 HO - 2 的表达水平分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Heme oxygenase (decycling) 2 ELISA Kit; Heme oxygenase (decyclizing) 2 ELISA Kit; Heme oxygenase 2 ELISA Kit; HMOX 2 ELISA Kit; Hmox2 ELISA Kit; HMOX2 protein ELISA Kit; HMOX2_HUMAN ELISA Kit; HO 2 ELISA Kit; HO-2 ELISA Kit; HO2 ELISA Kit; OTTHUMP00000159847 ELISA Kit
缩写：HMOX2
Uniprot No.：P30519
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HO-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 84-95 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 87-101 | ||||||
| 1:4 | Average % | 98 | |||||
| Range % | 94-104 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 93-105 | ||||||
回收率：
| The recovery of human HO-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 93-102 | |||||
| EDTA plasma (n=4) | 91 | 84-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.4472.2922.3702.222101.9591.8201.8901.74251.1611.2511.2061.0582.50.7060.7630.7350.5871.250.4060.4160.4110.2630.6250.2900.2980.2940.1460.3120.2210.2230.2220.07400.1440.1520.148 | |||||||
货期：3-5 working days
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