首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠4-羟基壬烯酸(HNE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的HNE含量。HNE即人中性粒细胞弹性蛋白酶，在炎症反应等生理病理过程中扮演重要角色。其过度表达与肺气肿、急性呼吸窘迫综合征等疾病密切相关。研究主要围绕抑制其活性展开，期望开发出能治疗相关疾病的药物，为临床治疗提供新方向。试剂盒检测范围为15.6 pmol/mL-1000 pmol/mL，本试剂盒可应用于氧化损伤相关研究领域，如代谢性疾病模型、心血管功能异常或神经退行性病变等科研场景中，通过检测血清或血浆样本的HNE水平，评估氧化应激程度及其在疾病进展中的作用机制，为实验动物模型的病理机制探索提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：HNE
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：15.6 pmol/mL-1000 pmol/mL
灵敏度：3.9 pmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 88-98 | |||||
回收率：
| The recovery of mouse HNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 89-97 | ||||
| EDTA plasma (n=4) | 94 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pmol/mlOD1OD2AverageCorrected10002.6082.5552.5822.3925001.9391.8971.9181.7282501.2271.2121.2201.0301250.8210.7590.7900.60062.50.4680.4500.4590.26931.20.3310.3280.3300.14015.60.2780.2710.2750.08500.1920.1880.190 | ||||||
货期：3-5 working days
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