首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Ⅲ型前胶原（HPCⅢ）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的HPCⅢ含量。小鼠Ⅲ型前胶原（HPCⅢ）是细胞外基质（ECM）的重要组成部分，其合成与降解失衡是肝纤维化的关键因素。砷暴露可诱导HPCⅢ表达增加，促进肝星状细胞（HSC）活化，导致ECM过度沉积，进而引发肝纤维化。研究揭示，HPCⅢ在肝纤维化发生发展中扮演着重要角色。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研中纤维化疾病机制或组织修复相关研究。该产品广泛应用于肝纤维化模型、肺纤维化模型或皮肤损伤修复等动物实验研究，为探索HPCⅢ在疾病发生发展中的作用或评价抗纤维化药物效果提供可靠工具，适用于体外实验环境下的浓度测定及动态变化分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：procollagen type 3 ,HPC3|procollagen type III ,HPCⅢ procollagen type III ,HPCIII
缩写：HPCⅢ
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
| 1:200 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:400 | Average % | 91 | |||||
| Range % | 85-95 | ||||||
| 1:800 | Average % | 102 | |||||
| Range % | 98-107 | ||||||
回收率：
| The recovery of mouse HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-93 | |||||
| EDTA plasma (n=4) | 84 | 80-88 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 10002.4922.3432.4182.316 5001.8251.7161.7711.669 2500.9471.0040.9760.874 1250.5710.5820.5770.475 62.50.3590.3390.3490.247 31.20.2460.2370.2420.140 15.60.1640.1710.1680.066 00.0980.1050.102 | |||||||
货期：3-5 working days
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