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产品描述：猪结合珠蛋白/触珠蛋白(Hpt/HP)酶联免疫试剂盒双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HP含量。试剂盒检测范围为1.56 μg/mL-100 μg/mL，其应用场景涵盖动物医学研究中的炎症标志物动态监测、疾病模型建立效果评估，以及畜牧养殖中猪群健康状态的分子水平研究，为探索Hpt/HP在病理生理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HP ELISA Kit; Haptoglobin [Cleaved into: Haptoglobin alpha chain; Haptoglobin beta chain] ELISA Kit
缩写：HP
Uniprot No.：Q8SPS7
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 μg/mL-100 μg/mL
灵敏度：0.39 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig Hpt/HP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 93 | ||||
| Range % | 89-98 | |||||
| 1:40 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:80 | Average % | 95 | ||||
| Range % | 89-98 | |||||
| 1:160 | Average % | 94 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of pig Hpt/HP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-102 | ||||
| EDTA plasma (n=4) | 87 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ug/mlOD1OD2AverageCorrected 1001.8121.7241.7681.625 501.1451.2121.1791.036 250.7150.7240.7200.577 12.50.4510.4380.4450.302 6.250.3120.3220.3170.174 3.120.2550.2420.2490.106 1.560.1960.1900.1930.050 00.1420.1440.143 | ||||||
货期：3-5 working days
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