首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：人乙酰肝素酶(HPA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、腹水、组织培养上清液、组织匀浆样本中的HPSE含量。HPSE即乙酰肝素酶，它在肿瘤生长、侵袭、转移及血管生成等过程中发挥重要作用。其研究机制主要围绕它可降解细胞外基质和基底膜中的硫酸乙酰肝素蛋白聚糖，从而促进肿瘤细胞的迁移和浸润，目前是肿瘤治疗的潜在靶点之一。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外研究HPA在肿瘤微环境调控、炎症性疾病机制或药物干预效果评估等科研场景，尤其适合处理复杂生物样本，如肿瘤患者腹水或组织样本分析，为探索HPA相关分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Endo glucoronidase ELISA Kit; Endo-glucoronidase ELISA Kit; HEP ELISA Kit; Heparanase 50 kDa subunit ELISA Kit; Heparanase ELISA Kit; Heparanase-1 ELISA Kit; Heparanase1 ELISA Kit; Hpa 1 ELISA Kit; HPA ELISA Kit; Hpa1 ELISA Kit; HPR 1 ELISA Kit; HPR1 ELISA Kit; HPSE 1 ELISA Kit; HPSE ELISA Kit; HPSE_HUMAN ELISA Kit; HPSE1 ELISA Kit; HSE 1 ELISA Kit; HSE1 ELISA Kit
缩写：HPSE
Uniprot No.：Q9Y251
种属：Homo sapiens (Human)
样本类型：serum, plasma, ascitic fluid, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human HPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 89-110 | ||||
| EDTA plasma (n=4) | 95 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.7612.7432.7522.636 52.4362.4242.4302.314 2.51.9021.9151.9091.793 1.251.2031.2191.2111.095 0.6250.6040.6120.6080.492 0.3120.3250.3200.3230.207 0.1560.2230.2310.2270.111 00.1120.1190.116 | ||||||
货期：3-5 working days
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