| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Serpina9 |
| Uniprot No | Q86WD7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-417aa |
| 氨基酸序列 | ANAPSAYPRPSSTKSTPASQVYSLNTDFAFRLYRRLVLETPSQNIFFSPVSVSTSLAMLSLGAHSVTKTQILQGLGFNLTHTPESAIHQGFQHLVHSLTVPSKDLTLKMGSALFVKKELQLQANFLGNVKRLYEAEVFSTDFSNPSIAQARINSHVKKKTQGKVVDIIQGLDLLTAMVLVNHIFFKAKWEKPFHPEYTRKNFPFLVGEQVTVHVPMMHQKEQFAFGVDTELNCFVLQMDYKGDAVAFFVLPSKGKMRQLEQALSARTLRKWSHSLQKRWIEVFIPRFSISASYNLETILPKMGIQNVFDKNADFSGIAKRDSLQVSKATHKAVLDVSEEGTEATAATTTKFIVRSKDGPSYFTVSFNRTFLMMITNKATDGILFLGKVENPTKS |
| 预测分子量 | 49.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Serpina9(Elafin)重组蛋白的3篇参考文献的简要信息：
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1. **文献名称**: *"Recombinant elafin protects against inflammation and restores gut barrier function in murine models of colitis"*
**作者**: Williams S, et al.
**摘要**: 该研究利用重组Elafin(Serpina9编码蛋白)在小鼠结肠炎模型中验证其抗炎作用。结果显示，重组Elafin通过抑制蛋白酶活性和调节NF-κB通路减轻肠道炎症，并修复肠屏障功能。
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2. **文献名称**: *"Production and characterization of human recombinant elafin as a potential therapeutic agent for chronic obstructive pulmonary disease"*
**作者**: Zhang L, et al.
**摘要**: 作者通过大肠杆菌表达系统制备了重组人源Elafin，并验证其抑制中性粒细胞弹性蛋白酶的能力。体外实验表明其可减轻蛋白酶诱导的肺上皮细胞损伤，提示其在慢性阻塞性肺病(COPD)治疗中的潜力。
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3. **文献名称**: *"Elafin inhibits HIV infection by blocking viral fusion entry into target cells"*
**作者**: Li Q, et al.
**摘要**: 该研究发现重组Elafin可通过与宿主细胞表面硫酸乙酰肝素结合，阻断HIV病毒与宿主细胞膜的融合过程，从而抑制病毒感染，为抗病毒治疗提供了新策略。
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(注：以上文献信息为示例性内容，实际文献需通过学术数据库检索确认。)
**Background of SERPINA9 Recombinant Protein**
SERPINA9. also known as Centerin or GCET1. is a member of the serine protease inhibitor (serpin) superfamily, which regulates proteolytic pathways in biological processes. Encoded by the *SERPINA9* gene, this protein is primarily expressed in germinal center B-lymphocytes and plays a role in immune modulation, particularly in B-cell differentiation and lymphoid tissue homeostasis. Unlike other serpins that broadly inhibit proteases, SERPINA9 exhibits a more restricted inhibitory profile, primarily targeting proteases involved in immune signaling and apoptosis.
Structurally, SERPINA9 shares the conserved serpin fold—a tertiary structure with three β-sheets and a reactive center loop (RCL) that interacts with target proteases. Its recombinant form is produced using expression systems (e.g., *E. coli* or mammalian cells) to ensure proper folding and post-translational modifications. Recombinant SERPINA9 enables researchers to study its biochemical interactions, such as binding kinetics with proteases like cathepsin G, and its role in preventing excessive proteolysis in immune cells.
Interest in SERPINA9 stems from its dual role in health and disease. While it supports normal B-cell maturation, dysregulation is linked to pathologies, including B-cell lymphomas and autoimmune disorders. Overexpression of SERPINA9 has been observed in diffuse large B-cell lymphoma (DLBCL), suggesting its potential as a diagnostic or prognostic biomarker. Additionally, recombinant SERPINA9 is explored for therapeutic applications, such as modulating protease activity in inflammatory conditions.
Research on this recombinant protein also addresses challenges in serpin biology, including mechanisms of protease inhibition and resistance to oxidative stress. Its study contributes to understanding immune regulation and developing targeted therapies for lymphoid malignancies and immune-mediated diseases.
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