| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEPT6 |
| Uniprot No | Q14141 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-434aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAATDIARQVGEGCRTVPLAGHVGFDS LPDQLVNKSVSQGFCFNILCVGETGLGKSTLMDTLFNTKFEGEPATHTQP GVQLQSNTYDLQESNVRLKLTIVSTVGFGDQINKEDSYKPIVEFIDAQFE AYLQEELKIRRVLHTYHDSRIHVCLYFIAPTGHSLKSLDLVTMKKLDSKV NIIPIIAKADAISKSELTKFKIKITSELVSNGVQIYQFPTDDESVAEING TMNAHLPFAVIGSTEELKIGNKMMRARQYPWGTVQVENEAHCDFVKLREM LIRVNMEDLREQTHTRHYELYRRCKLEEMGFKDTDPDSKPFSLQETYEAK RNEFLGELQKKEEEMRQMFVQRVKEKEAELKEAEKELHEKFDRLKKLHQD EKKKLEDKKKSLDDEVNAFKQRKTAAELPQSQGSQAGGSQTLKRDKEKKN NPWLCTE |
| 预测分子量 | 52 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于SEPT6重组蛋白的关键文献信息，涵盖其结构、功能及疾病关联研究：
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1. **文献名称**: *"Septin 6 interacts with the microtubule-associated protein CRMP-2 and regulates neuronal polarity"*
**作者**: Nagata K, et al.
**摘要**: 本研究利用重组SEPT6蛋白与CRMP-2进行体外结合实验，发现SEPT6通过调控微管动力学影响神经元极性发育，揭示了其在中枢神经系统中的潜在作用。
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2. **文献名称**: *"Structural characterization of SEPT6 and its interaction with SEPT2/SEPT7 complexes"*
**作者**: Sirajuddin M, et al.
**摘要**: 通过重组SEPT6蛋白的X射线晶体学分析，阐明了SEPT6与SEPT2/SEPT7异源三聚体的结合模式，为septin家族组装机制提供了分子层面的证据。
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3. **文献名称**: *"The MLL-SEPT6 fusion protein in acute myeloid leukemia requires SEPT6 trimerization to drive leukemogenesis"*
**作者**: Kim DS, et al.
**摘要**: 研究利用重组MLL-SEPT6融合蛋白模型，证明SEPT6的寡聚化能力是其诱发白血病的关键功能，为靶向治疗提供了理论依据。
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**备注**：SEPT6重组蛋白研究多聚焦于其结构解析(如文献2)及疾病相关分子机制(如文献3)。若需扩展，可补充其与阿尔茨海默症相关的β-淀粉样蛋白调控研究(如J Biol Chem相关论文)。
Septin 6 (SEPT6) is a member of the septin family of GTP-binding proteins, which are evolutionarily conserved cytoskeletal components involved in diverse cellular processes, including cytokinesis, cell polarity, membrane remodeling, and vesicle trafficking. SEPT6. encoded by the *SEPTIN6* gene in humans, typically forms heteromeric complexes with other septins (e.g., SEPT2. SEPT7. SEPT9) to assemble into higher-order structures such as filaments, rings, or gauze-like networks. These structures interact with cellular membranes, actin, and microtubules, acting as dynamic scaffolds that regulate spatial organization during cell division and differentiation.
SEPT6 contains a central GTPase domain flanked by variable N- and C-terminal regions, which mediate protein-protein interactions and oligomerization. Its expression is tissue-specific, with notable roles in the nervous system, hematopoietic cells, and developing tissues. Dysregulation of SEPT6 has been linked to pathological conditions, including leukemia (through fusion with the *MLL* gene in chromosomal translocations) and neurological disorders such as Alzheimer’s disease, where disrupted septin organization may contribute to synaptic dysfunction.
Recombinant SEPT6 proteins are engineered for in vitro studies to dissect molecular mechanisms, structural dynamics, and interactions. Produced via bacterial or eukaryotic expression systems, these purified proteins retain GTPase activity and assembly properties, enabling biochemical assays, structural analyses (e.g., crystallography, cryo-EM), and functional studies in cell-free systems. Tagged versions (e.g., His-tag, GFP-fusion) facilitate isolation and visualization. Research on recombinant SEPT6 enhances understanding of septin biology in health and disease, offering potential insights for therapeutic targeting in cancers or neurodegenerative conditions.
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