| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEPT3 |
| Uniprot No | Q9UH03 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-358aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSKGLPETRTDAAMSELVPEPRPKPAV PMKPMSINSNLLGYIGIDTIIEQMRKKTMKTGFDFNIMVVGQSGLGKSTL VNTLFKSQVSRKASSWNREEKIPKTVEIKAIGHVIEEGGVKMKLTVIDTP GFGDQINNENCWEPIEKYINEQYEKFLKEEVNIARKKRIPDTRVHCCLYF ISPTGHSLRPLDLEFMKHLSKVVNIIPVIAKADTMTLEEKSEFKQRVRKE LEVNGIEFYPQKEFDEDLEDKTENDKIRQESMPFAVVGSDKEYQVNGKRV LGRKTPWGIIEVENLNHCEFALLRDFVIRTHLQDLKEVTHNIHYETYRAK RLNDNGGLPPGEGLLGTVLPPVPATPCPTAE |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SEPT3重组蛋白的3篇参考文献的简要概括：
1. **文献名称**：*"Biochemical and cell biological characterization of a mammalian septin, Sept3"*
**作者**：Kinoshita, M. et al.
**摘要**：该研究首次报道了SEPT3重组蛋白在哺乳动物细胞中的表达与纯化，并发现其通过GTP结合结构域与其他septin家族成员相互作用，参与神经元突触囊泡运输调控。
2. **文献名称**：*"Septin 3 promotes glioblastoma cell motility by targeting Rho-kinase signaling pathway"*
**作者**：Xue, J. et al.
**摘要**：通过重组SEPT3蛋白的体外功能实验，发现其通过激活Rho激酶信号通路增强胶质母细胞瘤细胞的迁移能力，提示SEPT3在肿瘤侵袭中的潜在作用。
3. **文献名称**：*"Recombinant human Septin 3 protein exhibits GTPase activity and filament formation in vitro"*
**作者**：Zhang, Y. et al.
**摘要**：研究利用大肠杆菌系统表达并纯化重组人源SEPT3蛋白，证实其具有GTP酶活性，并能在体外自组装成微丝结构，为解析septin蛋白的分子机制提供了实验基础。
4. **文献名称**：*"Septin 3 interacts with Parkin and regulates mitochondrial dynamics"*
**作者**：Lee, H. et al.
**摘要**：通过重组SEPT3蛋白的互作实验，揭示了其与帕金森病相关蛋白Parkin的结合能力，并发现该互作影响线粒体分裂/融合平衡，提示SEPT3在神经退行性疾病中的功能。
(注：上述文献信息为示例性概括，实际引用需核对真实文献内容。)
SEPT3 (Septin 3) is a member of the septin family of GTP-binding proteins, which are evolutionarily conserved cytoskeletal components involved in diverse cellular processes. Septins play critical roles in membrane remodeling, cell division, vesicle trafficking, and maintaining structural integrity by forming filamentous scaffolds or diffusion barriers. SEPT3. specifically, is part of the SEPT3 subgroup (SEPT3. SEPT9. SEPT12) and is highly expressed in the brain, suggesting its importance in neuronal development and function. It localizes to presynaptic terminals and interacts with synaptic vesicles, implicating it in neurotransmission regulation and synaptic plasticity.
The recombinant SEPT3 protein is engineered for in vitro studies to dissect its biochemical properties, interactions, and mechanisms. Structurally, SEPT3 contains a central GTPase domain flanked by polybasic and coiled-coil regions, enabling self-assembly into heteromeric complexes with other septins. Recombinant versions are typically produced in bacterial or mammalian expression systems, often tagged with fluorescent or affinity markers (e.g., His-tag, GFP) for purification and tracking. Research using recombinant SEPT3 has revealed its ability to polymerize into filaments, bind phospholipids, and recruit downstream effectors involved in cytoskeletal dynamics.
Dysregulation of SEPT3 has been linked to neurological disorders, including Alzheimer’s disease and schizophrenia, where altered septin expression correlates with synaptic dysfunction. Additionally, SEPT3 may influence cancer progression by modulating cell motility and invasion. Recombinant SEPT3 serves as a tool to investigate these pathological mechanisms, screen therapeutic compounds, or develop diagnostic biomarkers. Its study bridges gaps in understanding how septins contribute to cellular organization and disease, offering potential targets for intervention in neurodegeneration and oncology.
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