| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEPSECS |
| Uniprot No | Q9HD40 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-501aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MNRESFAAGE RLVSPAYVRQ GCEARRSHEH LIRLLLEKGK CPENGWDEST LELFLHELAI MDSNNFLGNC GVGEREGRVA SALVARRHYR FIHGIGRSGD ISAVQPKAAG SSLLNKITNS LVLDIIKLAG VHTVANCFVV PMATGMSLTL CFLTLRHKRPKAKYIIWPRI DQKSCFKSMI TAGFEPVVIE NVLEGDELRT DLKAVEAKVQ ELGPDCILCI HSTTSCFAPR VPDRLEELAV ICANYDIPHIVNNAYGVQSS KCMHLIQQGA RVGRIDAFVQ SLDKNFMVPV GGAIIAGFND SFIQEISKMY PGRASASPSL DVLITLLSLG SNGYKKLLKE RKEMFSYLSN QIKKLSEAYN ERLLHTPHNP ISLAMTLKTL DEHRDKAVTQ GSMLFTRQV SGARVVPLGS MQTVSGYTFR GFMSHTNNYP CAYLNAASAI GMKMQDVDLF IKRLDRCLKA VRKERSKESD DNYDKTEDVD IEEMALKLDN VLLDTYQDAS S |
| 预测分子量 | 58 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于SEPSECS重组蛋白的参考文献，按文献名称、作者及摘要内容简要概括：
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1. **文献名称**：*Structural Insights into the Mechanism of the Eukaryotic Selenocysteine Incorporation Machinery*
**作者**：Palioura S. et al.
**摘要**：该研究解析了人源SEPSECS重组蛋白的晶体结构，揭示了其催化硒代半胱氨酸(Sec)从磷酸丝氨酸-tRNA(Sep-tRNA)转移至硒蛋白mRNA的分子机制，阐明其依赖ATP的构象变化及底物识别过程。
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2. **文献名称**：*Functional Characterization of Recombinant Human SEPSECS in Selenoprotein Synthesis*
**作者**：Xu X.M. et al.
**摘要**：通过在大肠杆菌中重组表达并纯化人源SEPSECS蛋白，验证其在体外催化Sep-tRNA转化为Sec-tRNA的活性，证实SEPSECS是硒代半胱氨酸生物合成通路中的关键酶，并依赖硒磷酸盐作为硒供体。
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3. **文献名称**：*SEPSECS Mutations Linked to Pontocerebellar Hypoplasia Disrupt tRNA Sec Interaction*
**作者**：Agamy O. et al.
**摘要**：研究利用重组SEPSECS蛋白进行突变分析，发现导致小脑发育异常的SEPSECS突变体(如R325W)会破坏其与tRNA Sec的结合能力，进而影响硒蛋白的合成及神经发育功能。
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如有需要进一步扩展或补充，请随时告知!
SEPSECS (Sep (O-Phosphoseryl) tRNA:Sec (Selenocysteine) tRNA synthase) is a key enzyme in selenocysteine (Sec) biosynthesis, a process essential for the incorporation of Sec into selenoproteins. Sec, often termed the 21st amino acid, is critical for the catalytic activity of selenoproteins involved in antioxidant defense, redox signaling, and thyroid hormone metabolism. Unlike standard amino acids, Sec is synthesized directly on its tRNA (tRNA^[Ser]Sec) through a multi-step process. SEPSECS catalyzes the final step, converting O-phosphoseryl-tRNA^[Ser]Sec to selenocysteinyl-tRNA^[Ser]Sec by replacing the phosphate group with selenium donated by selenophosphate. This reaction is vital for translational insertion of Sec into selenoproteins via a specialized UGA recoding mechanism.
Structurally, SEPSECS belongs to the fold-type Pyridoxal 5'-phosphate (PLP)-dependent enzyme family, though it uniquely functions without PLP. It forms a homodimer, with active sites that coordinate selenium transfer. Mutations in the SEPSECS gene are linked to pontocerebellar hypoplasia type 2D (PCH2D), a severe neurodevelopmental disorder characterized by cerebellar atrophy, intellectual disability, and early mortality. This underscores its non-redundant role in neural development.
Recombinant SEPSECS protein is produced using expression systems like E. coli or mammalian cells, enabling biochemical studies, structural analysis (e.g., X-ray crystallography), and therapeutic exploration. Its recombinant form aids in elucidating Sec biosynthesis mechanisms, modeling PCH2D pathologies, and screening potential drugs to restore enzyme activity in genetic disorders. Additionally, it serves as a tool for engineering selenoproteins or optimizing Sec incorporation in synthetic biology. Research on SEPSECS continues to bridge gaps in understanding selenium biology and its implications in neurodegeneration and oxidative stress-related diseases.
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