| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SERPINB2 |
| Uniprot No | P05120 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-415aa |
| 氨基酸序列 | MEDLCVANTLFALNLFKHLAKASPTQNLFLSPWSISSTMAMVYMGSRGSTEDQMAKVLQFNEVGANAVTPMTPENFTSCGFMQQIQKGSYPDAILQAQAADKIHSSFRSLSSAINASTGNYLLESVNKLFGEKSASFREEYIRLCQKYYSSEPQAVDFLECAEEARKKINSWVKTQTKGKIPNLLPEGSVDGDTRMVLVNAVYFKGKWKTPFEKKLNGLYPFRVNSAQRTPVQMMYLREKLNIGYIEDLKAQILELPYAGDVSMFLLLPDEIADVSTGLELLESEITYDKLNKWTSKDKMAEDEVEVYIPQFKLEEHYELRSILRSMGMEDAFNKGRANFSGMSERNDLFLSEVFHQAMVDVNEEGTEAAAGTGGVMTGRTGHGGPQFVADHPFLFLIMHKITNCILFFGRFSSP |
| 预测分子量 | 50.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SERPINB2重组蛋白的3篇参考文献(因文献检索时效性限制，以下为示例性内容，具体文献需通过学术数据库核实)：
1. **文献名称**: *"Recombinant SERPINB2 inhibits plasminogen activation and promotes cell adhesion in vitro"*
**作者**: Smith A, et al.
**摘要**: 研究报道了通过大肠杆菌系统成功表达重组SERPINB2蛋白，并证明其能有效抑制纤溶酶原激活剂(tPA)活性，同时发现该蛋白通过整合素途径增强细胞黏附功能。
2. **文献名称**: *"Structural and functional characterization of recombinant SERPINB2 in inflammatory regulation"*
**作者**: Lee J, et al.
**摘要**: 利用哺乳动物细胞表达系统获得高纯度SERPINB2重组蛋白，通过晶体结构解析和体外实验揭示其通过抑制尿激酶型纤溶酶原激活剂(uPA)调控巨噬细胞炎症反应的作用机制。
3. **文献名称**: *"Recombinant PAI-2 (SERPINB2) suppresses tumor metastasis via modulating extracellular matrix remodeling"*
**作者**: Chen H, et al.
**摘要**: 研究通过昆虫杆状病毒系统表达重组SERPINB2(PAI-2)，发现其通过抑制基质金属蛋白酶(MMPs)活性减少肿瘤细胞侵袭，并在小鼠模型中验证其抗转移效果。
**提示**：建议通过PubMed、Web of Science等平台以关键词“SERPINB2 recombinant”“PAI-2 expression”检索最新文献获取具体信息。
SERPINB2. also known as plasminogen activator inhibitor-2 (PAI-2), is a member of the serine protease inhibitor (serpin) superfamily. It functions as an inhibitor of urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA), enzymes critical for fibrinolysis and extracellular matrix remodeling. Unlike many serpins, SERPINB2 is primarily intracellular and lacks a classical secretion signal, though it can be secreted under specific conditions, such as inflammation or pregnancy. Its expression is induced by cytokines, growth factors, and cellular stress, linking it to processes like apoptosis, differentiation, and immune regulation.
Recombinant SERPINB2 is produced using expression systems like *E. coli* or mammalian cells, enabling studies of its structure and function. The protein’s reactive center loop (RCL) mediates protease inhibition through a unique "suicide substrate" mechanism, where cleavage triggers a conformational change, irreversibly inactivating the target protease. Recombinant forms are used to explore its roles in diseases, including cancer, thrombosis, and inflammatory disorders. For example, SERPINB2 is upregulated in certain tumors, where it may either promote survival by inhibiting apoptosis or suppress metastasis by modulating protease activity.
Clinically, SERPINB2 has been implicated in preeclampsia due to its elevated levels in maternal serum during pregnancy. Its recombinant variants are also investigated for therapeutic potential, such as modulating fibrinolysis or targeting uPA-dependent pathways in cancer. However, its dual roles in pro- and anti-inflammatory responses highlight context-dependent complexity. Research continues to unravel its interactions with viral proteases, immune cells, and signaling pathways, underscoring its importance in both basic biology and translational medicine.
×
