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产品描述：小鼠热休克蛋白HSP90β(HSP90AB1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的HSP90AB1含量。HSP90AB1，也称为HSP90β，是HSP90家族的一员，参与细胞信号转导、蛋白质折叠和降解等过程。研究发现，HSP90AB1在多种癌症中表达上调，其通过调节N蛋白稳定性影响病毒增殖，成为PDCoV致病机制的关键宿主因子。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，其宽泛的样本兼容性支持科研人员探究HSP90β在小鼠疾病模型（如肿瘤、神经退行性疾病）中的表达特征，或用于评估药物干预、热休克应激等条件下该蛋白的动态变化。实验操作流程标准化，配套完整试剂组分，适用于细胞生物学、免疫学及基础医学研究领域，为探索HSP90β的分子机制及其在病理生理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hsp90ab1 ELISA Kit; Hsp84 ELISA Kit; Hsp84-1 ELISA Kit; Hspcb ELISA Kit; Heat shock protein HSP 90-beta ELISA Kit; Heat shock 84 kDa ELISA Kit; HSP 84 ELISA Kit; HSP84 ELISA Kit; Tumor-specific transplantation 84 kDa antigen ELISA Kit; TSTA ELISA Kit
缩写：HSP90AB1
Uniprot No.：P11499
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HSP90AB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 85-98 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 81-100 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-105 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 85-102 | |||||
回收率：
| The recovery of mouse HSP90AB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 84-105 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.0142.1322.0731.8985001.3781.3551.3671.1922500.7480.7260.7370.5621250.4320.4220.4270.25262.50.2990.3090.3040.12931.250.2450.2530.2490.07415.60.2170.2270.2220.04700.1730.1760.175 | ||||||
货期：3-5 working days
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