首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠热休克蛋白70(HSP-70)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HSPA1A含量。HSPA1A 属于热休克蛋白 70 家族成员，在细胞应激反应中发挥重要作用。其表达异常与多种疾病如肿瘤发生发展相关。研究机制上，它能参与蛋白折叠、转运及降解等过程，还可调节细胞凋亡等通路，成为疾病治疗潜在靶点受到广泛关注。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外实验环境下检测小鼠模型中HSP - 70的动态变化，例如在热应激、氧化损伤或药物干预实验中评估组织或体液中HSP - 70的表达调控；适用于基础科研中细胞应激机制研究、疾病模型分析或生物标志物探索等场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hspa1a ELISA Kit; Hsp70-3 ELISA Kit; Hsp70A1Heat shock 70 kDa protein 1A ELISA Kit; Heat shock 70 kDa protein 3 ELISA Kit; HSP70.3 ELISA Kit; Hsp68 ELISA Kit
缩写：HSPA1A
Uniprot No.：Q61696
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hsp-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:40 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:80 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:160 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of mouse Hsp-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.7462.7982.7722.58452.0672.0692.0681.8802.51.3641.3321.3481.1601.250.7890.7560.7730.5850.6250.5640.5720.5680.3800.3120.4330.4320.4330.2450.1560.2810.2680.2750.08700.1870.1890.188 | ||||||
货期：3-5 working days
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