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品描述：山羊热休克蛋白70(HSP-70)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HSPA2含量。HSPA2，全称为热休克蛋白A2，是HSPA（HSP70）分子伴侣家族的一员，对男性生育能力具有关键作用。存在于多种体细胞器官中，可能参与调节上皮细胞分化，同时作为癌症相关蛋白，其功能意义尚未达成共识。研究显示，HSPA2在细胞蛋白质质量控制中起核心作用，保护蛋白质组，协助蛋白质折叠和运输，激活蛋白水解，并调节蛋白质复合物形成。试剂盒检测范围为23.44 ng/mL-1500 ng/mL，在科研领域，可用于探究环境胁迫、病原感染或代谢异常等因素对山羊HSP - 70表达的影响，亦适用于动物模型研究、生理机制解析及免疫调控相关实验，为畜牧科学、兽医学及基础生命科学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HSPA2 ELISA Kit; HSP70-3 ELISA Kit; Heat shock-related 70 kDa protein 2 ELISA Kit; Heat shock 70 kDa protein 3 ELISA Kit; HSP70.3 ELISA Kit
缩写：HSPA2
Uniprot No.：Q9TUG3
种属：Capra hircus (Goat)
样本类型：serum, plasma, tissue homogenates
检测范围：23.44 ng/mL-1500 ng/mL
灵敏度：5.86 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-103 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of goat HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-98 | ||||
| EDTA plasma (n=4) | 95 | 89-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected15002.3082.4632.3862.2347501.6061.6661.6361.4843751.0781.0641.0710.919187.50.7320.7210.7270.57593.750.4280.4560.4420.29046.880.3130.3300.3220.17023.440.2190.2350.2270.07500.1510.1530.152 | ||||||
货期：3-5 working days
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