首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人热休克蛋白β-2(HSPB2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HSPB2含量。HSPB2属于小分子热休克蛋白家族。其背景上，它在多种组织中表达，与细胞应激保护、凋亡调控等密切相关。研究机制方面，主要通过与其他蛋白相互作用来稳定蛋白结构、调节细胞信号通路，参与维持细胞内环境稳定，相关研究聚焦其在疾病发生发展中的作用。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础科研中探究HSPB2在应激反应调控、疾病模型构建或药物干预实验中的动态表达，例如阿尔茨海默病、心肌缺血等疾病机制研究，或作为生物标志物在相关分子通路分析中的应用本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DMPK-binding protein ELISA Kit; Heat shock 27kD protein 2 ELISA Kit; Heat shock 27kDa protein 2 ELISA Kit; Heat shock protein beta-2 ELISA Kit; Heat-shock protein beta-2 ELISA Kit; Hs.78846 ELISA Kit; HSP27 ELISA Kit; HspB2 ELISA Kit; HSPB2_HUMAN ELISA Kit; LOH11CR1K ELISA Kit; MGC133245 ELISA Kit; MKBP ELISA Kit
缩写：HSPB2
Uniprot No.：Q16082
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HSPB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 107 | |||||
| Range % | 100-111 | ||||||
| 1:2 | Average % | 99 | |||||
| Range % | 91-103 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 87-100 | ||||||
| 1:8 | Average % | 102 | |||||
| Range % | 94-107 | ||||||
回收率：
| The recovery of human HSPB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 89-93 | |||||
| EDTA plasma (n=4) | 98 | 94-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.5262.6012.5642.405102.1152.0562.0861.92751.4171.4031.4101.2512.50.8380.8540.8460.6871.250.5030.4890.4960.3370.6250.3250.3090.3170.1580.3120.2450.2340.2400.08100.1610.1570.159 | |||||||
货期：3-5 working days
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