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Human chaperonin 10,CPN10 ELISA Kit

  • 中文名： 人伴侣蛋白10(CPN10)ELISA Kit
货号: IPD-E09917h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人伴侣蛋白10(CPN10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HSPE1含量。HSPE1是一个值得关注的靶点。它在细胞生理活动中发挥重要作用，与细胞应激反应、蛋白质折叠等相关。研究机制主要聚焦于其在疾病发生发展中的功能，如在肿瘤细胞增殖、存活等过程的作用，通过调控它或能找到新治疗策略。试剂盒检测范围为0.16 ng/mL-10 ng/mL，适用于体外检测CPN10在细胞应激机制研究、疾病模型（如神经退行性疾病或代谢综合征）中的动态表达分析，以及蛋白质相互作用的基础科研领域，为探索CPN10生物学功能提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    10 kDa chaperonin ELISA Kit; 10 kDa heat shock protein mitochondrial ELISA Kit; 10 kDa heat shock protein; mitochondrial ELISA Kit; CH10_HUMAN ELISA Kit; Chaperonin 10 ELISA Kit; Chaperonin 10 homolog ELISA Kit; CPN10 ELISA Kit; cpn10 homolog ELISA Kit; Early pregnancy factor ELISA Kit; Early-pregnancy factor ELISA Kit; EPF ELISA Kit; GROES ELISA Kit; GroES homolog ELISA Kit; Heat shock 10kD protein 1 chaperonin 10 ELISA Kit; Heat shock 10kDa protein 1 ELISA Kit; Heat shock 10kDa protein 1 chaperonin 10 ELISA Kit; Heat shock protein family E (Hsp10) member ELISA Kit; Heat-shock 10-kD protein ELISA Kit; Hsp10 ELISA Kit; Hspe1 ELISA Kit
  • 缩写：
    HSPE1
  • Uniprot No.：
    P61604
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.16 ng/mL-10 ng/mL
  • 灵敏度：
    0.04 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%



    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%



    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CPN10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)

    1:1Average %92

    Range %88-94

    1:2Average %102

    Range %97-107

    1:4Average %94

    Range %87-98

    1:8Average %102

    Range %98-106

  • 回收率：
    The recovery of human CPN10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange

    Serum (n=5)8983-94

    EDTA plasma (n=4)9287-96

















    标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.









































































































    ng/mlOD1OD2AverageCorrected

    102.6882.5792.6342.525

    52.1542.0542.1041.995

    2.51.3651.3021.3341.225

    1.250.7420.7340.7380.629

    0.630.4170.4050.4110.302

    0.310.2740.2660.2700.161

    0.160.1860.1830.1850.076

    00.1100.1080.109











  • 货期：
    3-5 working days


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