首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人热休克蛋白60(Hsp-60)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HSPD1含量。HSPD1，即热休克蛋白60（HSP60）基因，编码一种在线粒体中发挥重要作用的伴侣蛋白。其研究机制涉及线粒体蛋白质质量控制、细胞凋亡、炎症和免疫反应等生物学功能，与多种神经系统疾病如遗传痉挛性截瘫、帕金森病等密切相关。试剂盒检测范围为1.56 ng/mL-100 ng/mL，该产品适用于基础医学研究、疾病机制探索及药物开发过程中Hsp - 60相关生物标志物分析，如心血管疾病模型、糖尿病并发症研究或神经退行性病变的分子病理学评估等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：60 kDa chaperonin ELISA Kit; 60 kDa heat shock protein; mitochondrial ELISA Kit; CH60_HUMAN ELISA Kit; Chaperonin 60 ELISA Kit; Chaperonin; 60-KD ELISA Kit; CPN60 ELISA Kit; fa04a05 ELISA Kit; GROEL ELISA Kit; heat shock 60kDa protein 1 (chaperonin) ELISA Kit; Heat shock protein 1 (chaperonin) ELISA Kit; Heat shock protein 60 ELISA Kit; Heat shock protein 65 ELISA Kit; heat shock protein family D (Hsp60) member 1 ELISA Kit; HLD4 ELISA Kit; Hsp 60 ELISA Kit; HSP 65 ELISA Kit; HSP-60 ELISA Kit; HSP60 ELISA Kit; HSP65 ELISA Kit; HSPD1 ELISA Kit; HuCHA60 ELISA Kit; Mitochondrial matrix protein P1 ELISA Kit; P60 lymphocyte protein ELISA Kit; short heat shock protein 60 Hsp60s1 ELISA Kit; SPG13 ELISA Kit
缩写：HSPD1
Uniprot No.：P10809
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HSP-60 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 88-99 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 83-96 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-84 | |||||
回收率：
| The recovery of human HSP-60 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 82-90 | ||||
| EDTA plasma (n=4) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.8212.8022.8122.690501.8861.7661.8261.704251.2361.2321.2341.11212.50.7190.7020.7110.5896.250.3830.3780.3810.2593.120.2930.2780.2860.1641.560.1900.1780.1840.06200.1250.1190.122 | ||||||
货期：3-5 working days
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