首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人硫酸乙酰肝素蛋白多糖2(HSPG2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的HSPG2含量。HSPG2 即硫酸乙酰肝素蛋白聚糖 2，是细胞外基质的重要成分。其在多种生理和病理过程中发挥作用，如组织发育和肿瘤进展等。研究机制方面，主要围绕其与生长因子结合、调节信号通路，影响细胞增殖、迁移和血管生成等展开。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于科研领域探究HSPG2在心血管疾病、肿瘤微环境调控中的作用机制，或作为基础研究中的生物标志物分析工具，亦可用于评估靶向治疗药物对HSPG2相关通路的影响，为体外实验提供精准的蛋白定量支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Basement membrane specific heparan sulfate proteoglycan core protein ELISA Kit; Endorepellin (domain V region) ELISA Kit; Heparan sulfate proteoglycan of basement membrane ELISA Kit; HSPG 2 ELISA Kit; HSPG ELISA Kit; Hspg2 ELISA Kit; LG3 peptide ELISA Kit; Perlecan ELISA Kit; PGBM_HUMAN ELISA Kit; PLC ELISA Kit; Schwartz Jampel syndrome 1 (chondrodystrophic myotonia) ELISA Kit; SJA ELISA Kit; SJS ELISA Kit; SJS1 ELISA Kit
缩写：HSPG2
Uniprot No.：P98160
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HSPG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 102 | |||||
| Range % | 89-107 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 91-102 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 84-98 | ||||||
| 1:8 | Average % | 106 | |||||
| Range % | 96-110 | ||||||
回收率：
| The recovery of human HSPG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 93-106 | |||||
| EDTA plasma (n=4) | 103 | 94-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40002.1712.0892.1301.97820001.5301.4831.5071.35510000.9250.9460.9360.7845000.5780.5650.5720.4202500.3520.3670.3600.2081250.2450.2530.2490.09762.50.2030.2080.2060.05400.1540.1500.152 | |||||||
货期：3-5 working days
×
