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产品描述：人丝氨酸蛋白酶HTRA1(HTRA1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的HTRA1含量。HTRA1，全称为高温需求丝氨酸蛋白酶A1，是一种丝氨酸蛋白酶，具有多种靶标，包括细胞外基质蛋白如纤连蛋白。它在细胞外基质重塑中发挥作用，与年龄相关性黄斑变性（AMD）和脑小血管病CARASIL综合征等相关。HTRA1的异常表达与AMD患者脉络膜新生血管过度增生密切相关，其蛋白酶活性调控机制成为潜在治疗靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于基础科研领域，可用于探究HTRA1在疾病发生发展中的分子机制、评估体外细胞模型中HTRA1的表达水平，或分析不同处理条件下生物样本中HTRA1的动态变化，为研究其在组织重塑、血管生成及神经退行性疾病中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ARMD7 ELISA Kit; CARASIL ELISA Kit; High-temperature requirement A serine peptidase 1 ELISA Kit; HtrA ELISA Kit; HtrA serine peptidase 1 ELISA Kit; HTRA1 ELISA Kit; HTRA1_HUMAN ELISA Kit; IGFBP5 protease ELISA Kit; L56 ELISA Kit; ORF480 ELISA Kit; Protease serine 11 (IGF binding) ELISA Kit; protease serine 11 ELISA Kit; PRSS11 ELISA Kit; Serine protease 11 ELISA Kit; Serine protease HTRA1 ELISA Kit; Serine protease HTRA1 precursor ELISA Kit
缩写：HTRA1
Uniprot No.：Q92743
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HTRA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 84-96 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 82-93 | |||||
回收率：
| The recovery of human HTRA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 84-105 | ||||
| EDTA plasma (n=4) | 98 | 92-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.3572.4052.3812.282502.1122.2122.1622.063251.8421.7001.7711.67212.51.2751.2951.2851.1866.250.8230.8880.8560.7573.120.4810.5030.4920.3931.560.2580.2630.2610.16200.1030.0950.099 | ||||||
货期：3-5 working days
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