首页 / 产品 / 蛋白 / 信号通路蛋白

Recombinant Human SFRP2 protein

  • 中文名: 分泌型卷曲相关蛋白2(SFRP2)重组蛋白
  • 别    名: SFRP2;FRP2;SARP1;Secreted frizzled-related protein 2
货号: PA1000-2900
Price: ¥询价
数量:
大包装询价

产品详情

纯度>90%SDS-PAGE.
种属Human
靶点SFRP2
Uniprot No Q96HF1
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间68-186aa
氨基酸序列TMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRFPQDNDLCIPLASSDHLLPATEEAPKVCEACKNKNDDDNDIM
预测分子量 44.9 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3篇关于SFRP2重组蛋白的假设参考文献(基于领域内常见研究方向模拟,实际文献需核实):

1. **"Secreted Frizzled-related Protein 2 (SFRP2) inhibits Wnt signaling and suppresses colorectal cancer cell growth"**

- 作者:Jones AB, et al.

- 摘要:研究利用重组SFRP2蛋白体外处理结直肠癌细胞,发现其通过结合Wnt配体阻断经典Wnt/β-catenin通路,显著抑制癌细胞增殖并诱导凋亡,提示SFRP2作为潜在肿瘤抑制因子。

2. **"Recombinant SFRP2 promotes angiogenesis and accelerates wound healing in diabetic mouse models"**

- 作者:Chen L, et al.

- 摘要:通过重组SFRP2蛋白局部给药,发现其通过激活非经典Wnt/Ca²⁺通路增强内皮细胞迁移和血管生成,显著改善糖尿病小鼠皮肤缺损模型的伤口愈合速度。

3. **"Structural and functional analysis of SFRP2 reveals its dual role in modulating Wnt signaling"**

- 作者:Wang Y, et al.

- 摘要:通过重组表达和X射线晶体学解析SFRP2蛋白结构,结合体外功能实验,揭示其通过不同结构域同时拮抗Wnt1和增强Wnt5a信号的双重调控机制。

4. **"SFRP2 recombinant protein enhances cardiac repair after myocardial infarction via modulation of macrophage polarization"**

- 作者:Kim T, et al.

- 摘要:动物实验表明,注射重组SFRP2蛋白可促进心肌梗死后M2型巨噬细胞极化,减少炎症反应并增加心肌细胞存活率,为心血管再生治疗提供新策略。

注:以上内容综合了SFRP2在癌症、组织修复和信号调控中的典型研究方向,实际文献需通过PubMed或Google Scholar检索关键词"SFRP2 recombinant protein"获取。

背景信息

**Background of SFRP2 Recombinant Protein**

Secreted Frizzled-related protein 2 (SFRP2) belongs to the SFRP family, a group of glycoproteins that modulate Wnt signaling—a critical pathway regulating embryogenesis, cell proliferation, and tissue homeostasis. SFRPs act as soluble antagonists by binding directly to Wnt ligands or Frizzled receptors, preventing their interaction and downstream signaling. SFRP2. in particular, contains a cysteine-rich domain (CRD) homologous to the extracellular Wnt-binding region of Frizzled receptors, enabling its competitive inhibitory function.

Biologically, SFRP2 plays dual roles depending on cellular context. It is implicated in embryonic development, bone formation, and angiogenesis. However, dysregulated SFRP2 expression is associated with pathologies, including cancer, fibrosis, and cardiovascular diseases. In cancer, SFRP2 exhibits paradoxical behavior: it can suppress Wnt/β-catenin signaling to inhibit tumor growth in some contexts, while promoting angiogenesis and tumor progression in others, likely through non-canonical pathways.

Recombinant SFRP2 protein is produced using expression systems like *E. coli* or mammalian cells, ensuring proper folding and post-translational modifications for functional studies. This protein is widely used in research to elucidate SFRP2’s mechanisms in development and disease, screen for therapeutic agents, or explore its potential as a biomarker. In regenerative medicine, SFRP2’s role in modulating Wnt signaling has sparked interest in applications for bone repair and tissue engineering.

Current studies focus on its therapeutic targeting—either by leveraging its anti-Wnt activity in cancers driven by hyperactive Wnt signaling or by blocking its pro-angiogenic effects in tumor microenvironments. Challenges remain in understanding its context-dependent functions, but recombinant SFRP2 remains a vital tool for decoding its biological complexity and translational potential.

客户数据及评论

折叠内容

大包装询价

×