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Recombinant Human SET protein

  • 中文名: 蛋白SET亚型1(SET)重组蛋白
  • 别    名: SET;Protein SET
货号: PA1000-2896
Price: ¥询价
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点SET
Uniprot NoQ01105
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-290aa
氨基酸序列MGSSHHHHHH SSGLVPRGSH MGSMAPKRQS PLPPQKKKPR PPPALGPEET SASAGLPKKG EKEQQEAIEH IDEVQNEIDR LNEQASEEIL KVEQKYNKLR QPFFQKRSEL IAKIPNFWVT TFVNHPQVSA LLGEEDEEAL HYLTRVEVTE FEDIKSGYRI DFYFDENPYF ENKVLSKEFH LNESGDPSSK STEIKWKSGK DLTKRSSQTQ NKASRKRQHE EPESFFTWFT DHSDAGADEL GEVIKDDIWP NPLQYYLVPD MDDEEGEGEE DDDDDEEEEG LEDIDEEGDE DEGEEDEDDD EGEEGEEDEG EDD
预测分子量36 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于SET重组蛋白的3篇示例参考文献(注:以下为模拟示例,实际文献需通过学术数据库检索确认):

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1. **文献名称**: "Structural Insights into the SET Domain-Mediated Methyltransferase Activity in Recombinant SET Protein"

**作者**: Zhang, L., et al.

**摘要**: 本研究解析了重组表达的SET蛋白结构域晶体结构,揭示了其介导组蛋白H3K27甲基化的分子机制,为设计靶向SET甲基转移酶活性的抑制剂提供了结构基础。

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2. **文献名称**: "Overexpression of Recombinant SET Oncoprotein Disrupts PP2A Activity and Promotes Leukemogenesis"

**作者**: Moreno, S., et al.

**摘要**: 通过体外重组SET蛋白实验,发现其过表达通过抑制蛋白磷酸酶PP2A的活性,导致细胞周期失调和急性髓系白血病进展,提示SET可作为白血病治疗的潜在靶点。

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3. **文献名称**: "Recombinant SET Protein Enhances Tau Hyperphosphorylation in Alzheimer's Disease Models"

**作者**: Kim, H., & Park, J.

**摘要**: 利用重组SET蛋白进行体外结合实验,证实其直接与Tau蛋白相互作用并促进病理性磷酸化,为阿尔茨海默病中神经退行性病变机制提供了新解释。

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如需真实文献,建议通过PubMed或Google Scholar以关键词“SET recombinant protein”、“SET domain function”或“SET oncoprotein”检索最新研究。

背景信息

SET (Suvar3-9. Enhancer of zeste, Trithorax) recombinant proteins are engineered versions of a critical family of epigenetic regulators involved in post-translational modifications, particularly histone methylation. Initially identified in Drosophila for their roles in chromatin remodeling and gene silencing, SET-domain-containing proteins are evolutionarily conserved across eukaryotes. The SET domain, a ~130-amino acid motif, catalyzes the transfer of methyl groups to specific lysine residues on histones (e.g., H3K9. H4K20), modulating chromatin structure and transcriptional activity. Dysregulation of SET proteins is linked to cancers, neurodegenerative disorders, and developmental anomalies due to aberrant epigenetic signaling.

Recombinant SET proteins are produced via heterologous expression systems (e.g., E. coli, yeast, or mammalian cells) to study their biochemical properties, substrate specificity, and interactions. Their production enables structural analysis (e.g., crystallography, cryo-EM) and high-throughput screening for inhibitors, aiding drug discovery. For instance, SETD7 (SET7/9), a histone H3K4 methyltransferase, is studied recombinantly to explore its role in cell cycle regulation and inflammation. Similarly, SETD2. which trimethylates H3K36. is linked to DNA repair mechanisms and cancer progression.

Challenges in producing functional SET recombinant proteins include maintaining proper folding and enzymatic activity, as methylation requires precise cofactor binding (e.g., S-adenosylmethionine). Fusion tags (e.g., GST, His-tag) are often added to enhance solubility and purification. Despite these hurdles, recombinant SET proteins remain indispensable tools for deciphering epigenetic pathways and developing targeted therapies. Recent advances in cryo-EM and AI-driven protein engineering are refining their production and application, highlighting their growing relevance in precision medicine and molecular biology research.

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