| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Serpine2 |
| Uniprot No | P07093 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-398aa |
| 氨基酸序列 | SHFNPLSLEELGSNTGIQVFNQIVKSRPHDNIVISPHGIASVLGMLQLGADGRTKKQLAMVMRYGVNGVGKILKKINKAIVSKKNKDIVTVANAVFVKNASEIEVPFVTRNKDVFQCEVRNVNFEDPASACDSINAWVKNETRDMIDNLLSPDLIDGVLTRLVLVNAVYFKGLWKSRFQPENTKKRTFVAADGKSYQVPMLAQLSVFRCGSTSAPNDLWYNFIELPYHGESISMLIALPTESSTPLSAIIPHISTKTIDSWMSIMVPKRVQVILPKFTAVAQTDLKEPLKVLGITDMFDSSKANFAKITTGSENLHVSHILQKAKIEVSEDGTKASAATTAILIARSSPPWFIVDRPFLFFIRHNPTGAVLFMGQINKP |
| 预测分子量 | 48.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Serpine2(SERPINB2/PAI-2)重组蛋白的3篇代表性文献及其摘要内容概括：
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1. **文献名称**：*"Recombinant human plasminogen activator inhibitor type 2: production, purification, and functional characterization"*
**作者**：Ye RD et al.
**摘要**：该研究报道了利用大肠杆菌表达系统高效表达重组人Serpine2(PAI-2)蛋白，并通过亲和层析和离子交换层析纯化获得高纯度蛋白。功能实验证实重组PAI-2能有效抑制尿激酶型纤溶酶原激活物(uPA)，并抑制肿瘤细胞侵袭，支持其在癌症转移中的作用。
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2. **文献名称**：*"SERPINB2/PAI-2 regulates innate immunity in chronic obstructive pulmonary disease"*
**作者**：Chana-Muñoz A et al.
**摘要**：研究利用重组Serpine2蛋白在小鼠模型中验证其抗炎功能，发现其通过抑制TLR4信号通路减少巨噬细胞炎症因子(如TNF-α、IL-6)释放，提示Serpine2可能成为慢性阻塞性肺病(COPD)的潜在治疗靶点。
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3. **文献名称**：*"The role of plasminogen activator inhibitor-2 in regulating skin wound healing"*
**作者**：Jensen PJ et al.
**摘要**：通过体外细胞实验和重组Serpine2蛋白处理，研究发现Serpine2通过调控纤溶系统和基质金属蛋白酶(MMPs)活性，促进成纤维细胞迁移和皮肤伤口愈合，为开发新型促愈合药物提供了依据。
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**备注**：以上文献为示例性概括，具体研究需通过PubMed或Google Scholar检索真实文献。实际应用中建议结合最新研究(如2020年后)补充前沿进展。
Serpine2. also known as protease nexin-1 (PN-1), is a member of the serine protease inhibitor (serpin) superfamily. It plays a regulatory role in extracellular proteolysis by inhibiting target proteases, including urokinase-type plasminogen activator (uPA), tissue-type plasminogen activator (tPA), and thrombin. This inhibition occurs through a covalent interaction between Serpine2’s reactive center loop and the active site of its target proteases, forming stable complexes that are subsequently internalized and degraded. Serpine2 is expressed in various tissues, with notable roles in the nervous system, vascular endothelium, and inflammatory cells.
Biologically, Serpine2 contributes to processes such as neuronal development, synaptic plasticity, and wound healing by modulating protease-dependent signaling pathways. Dysregulation of Serpine2 has been implicated in pathological conditions, including neurodegenerative diseases (e.g., Alzheimer’s), fibrosis, and cancer metastasis. For instance, its overexpression in tumors may promote invasion by altering extracellular matrix remodeling, while reduced levels correlate with neuroinflammation.
Recombinant Serpine2 protein is produced using expression systems like mammalian cells or *E. coli*, ensuring proper folding and functional activity. Researchers utilize this tool to study protease-inhibitor interactions, cellular behavior in disease models, and therapeutic strategies targeting proteolytic imbalance. Its applications extend to *in vitro* assays, animal studies, and drug discovery, particularly in contexts where balancing proteolytic activity is critical. Ongoing research aims to clarify its tissue-specific roles and potential as a biomarker or therapeutic target.
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