| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SERPINB3 |
| Uniprot No | P29508 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-390aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMNSLSEANTKFMFDLFQQFRKSKENNI FYSPISITSALGMVLLGAKDNTAQQIKKVLHFDQVTENTTGKAATYHVDR SGNVHHQFQKLLTEFNKSTDAYELKIANKLFGEKTYLFLQEYLDAIKKFY QTSESVDFANAPEESRKKINSWVESQTNEKIKNLIPEGNIGSNTTLVLNA IYFKGQWEKKFNKEDTKEEKFWPNKNTYKSIQMMRQYTSFHFASLEDVQA KVLEIPYKGKDLSMIVLLPNEIDGLQKLEEKLTAEKLMEWTSLQNMRETR VDLHLPRFKVEESYDLKDTLRTMGMVDIFNGDADLSGMTGSRGLVLSGVL HKAFVEVTEEGAEAAAATAVVGFGSSPTSTNEEFHCNHPFLFFIRQNKTN SILFYGRFSSP |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"SERPINB3 modulates autophagy and oxidative stress in chronic liver disease" by Vidali et al.**
摘要：研究通过重组SERPINB3蛋白揭示了其在肝纤维化中的作用，表明其通过调节自噬和氧化应激途径影响疾病进展，可能成为治疗靶点。
2. **"Recombinant SERPINB3 inhibits cysteine proteases and promotes tumor cell survival" by Pontisso et al.**
摘要：利用重组SERPINB3蛋白验证其对半胱氨酸蛋白酶(如组织蛋白酶)的抑制作用，发现其通过阻断凋亡通路增强肿瘤细胞存活率，与癌症耐药性相关。
3. **"SERPINB3 overexpression enhances epithelial-mesenchymal transition in head and neck cancer" by Catanzaro et al.**
摘要：通过重组蛋白实验证实SERPINB3过表达促进上皮-间质转化(EMT)，导致癌细胞侵袭性增强，提示其在转移中的关键作用。
4. **"Role of SERPINB3 in psoriasis: Insights from recombinant protein studies" by Zhou et al.**
摘要：重组SERPINB3蛋白实验表明其通过抑制角质形成细胞中的蛋白酶活性，加剧皮肤炎症反应，可能参与银屑病病理机制。
(注：以上文献信息为模拟示例，实际引用请查询PubMed等数据库获取真实文献。)
SERPINB3. also known as squamous cell carcinoma antigen 1 (SCCA1), is a member of the serine protease inhibitor (serpin) superfamily. It is primarily expressed in epithelial tissues and plays a regulatory role in protease-mediated processes, including inflammation, apoptosis, and tissue remodeling. Structurally, SERPINB3 contains a conserved reactive center loop (RCL) that interacts with target proteases, inhibiting their activity through a unique "suicide substrate" mechanism. Unlike classical serpins that inhibit serine proteases, SERPINB3 primarily targets cysteine proteases, such as cathepsins, which are involved in extracellular matrix degradation and immune response modulation.
This protein has garnered attention due to its dual role in both protective and pathological contexts. Under normal conditions, SERPINB3 acts as a stress-response protein, safeguarding cells against oxidative damage and apoptosis. However, its overexpression is strongly linked to several diseases, including squamous cell carcinomas, chronic liver diseases (e.g., fibrosis and cirrhosis), and autoimmune disorders. In cancer, elevated SERPINB3 levels correlate with tumor progression, metastasis, and resistance to therapies, likely by promoting cell survival and evading immune surveillance.
Recombinant SERPINB3 protein is engineered for research and therapeutic applications. It is typically produced using expression systems like *E. coli* or mammalian cells, ensuring proper folding and post-translational modifications. Researchers utilize this recombinant form to study SERPINB3's mechanistic roles in disease models, screen for inhibitory compounds, or develop diagnostic assays. Additionally, it serves as a potential biomarker for monitoring disease progression or therapeutic efficacy. Despite its clinical associations, the precise molecular pathways regulated by SERPINB3 remain under investigation, highlighting the need for further studies to exploit its therapeutic potential while mitigating its pathological effects.
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