首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠羟脯氨酸(Hyp)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Hyp含量。试剂盒检测范围为7.8 ng/mL-500 ng/mL，在纤维化疾病研究、组织修复机制探索及骨骼发育相关科研领域具有重要价值；适用于体外培养模型、动物实验样本中胶原代谢的动态监测；为抗纤维化药物筛选、组织工程研究及代谢性疾病机制分析提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Hyp
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hyp in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 89 | ||||
| Range % | 82-97 | |||||
| 1:40 | Average % | 98 | ||||
| Range % | 91-104 | |||||
| 1:80 | Average % | 98 | ||||
| Range % | 96-105 | |||||
| 1:160 | Average % | 97 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of mouse Hyp spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-101 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected5002.6562.7552.7062.5532502.0882.1872.1381.9851251.4921.5181.5051.35262.50.8410.8500.8460.69331.250.5060.5150.5110.35815.60.3370.3490.3430.1907.80.2620.2710.2670.11400.1520.1530.153 | ||||||
货期：3-5 working days
×
