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产品描述：人磷酸化Tau蛋白酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、脑脊液（CSF）样本中的pMAPT /pTAU含量。pMAPT/pTAU作为靶点，在神经退行性疾病如阿尔茨海默病背景下备受关注。它与微管相关蛋白tau异常磷酸化有关。研究机制围绕探索其磷酸化调控过程、异常磷酸化后对神经细胞功能影响，期望以此为突破点开发疾病治疗手段。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品可广泛应用于神经退行性疾病机制研究、磷酸化Tau蛋白动态变化监测、药物干预效果评估等科研领域，为探索病理过程中生物标志物变化提供可靠工具，尤其适用于阿尔茨海默病模型研究、体液生物标志物筛查及神经科学相关基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：pMAPT /pTAU
种属：Homo sapiens (Human)
样本类型：serum, plasma, cerebrospinal fluid (CSF)
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human pMAPT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 81-92 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 96-108 | |||||
回收率：
| The recovery of human pMAPT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 98 | 93-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.2782.1842.2312.05910001.7811.7251.7531.5815001.2681.2111.2401.0682500.7160.7420.7290.5571250.5850.5370.5610.38962.50.3690.3490.3590.18731.250.2540.2720.2630.09100.1740.1690.172 | ||||||
货期：3-5 working days
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