首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胰岛淀粉样多肽(IAPP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液样本中的IAPP含量。IAPP即胰岛淀粉样多肽，是胰岛β细胞分泌的一种激素。与糖尿病关系密切，其异常聚集形成的淀粉样沉积物会损伤胰岛β细胞，影响胰岛素分泌。目前对它的研究多聚焦于抑制其聚集、减少细胞毒性等机制，以探索治疗糖尿病新策略。试剂盒检测范围为47 pg/mL-3000 pg/mL，适用于探索糖尿病发病机制、评估药物干预对IAPP表达的影响，以及代谢综合征动物模型的生物标志物分析等领域。其兼容多种生物体液样本的特点，尤其适合开展临床前研究或非侵入性样本检测，为体外实验和基础研究提供可靠的数据支持，满足科研工作者对IAPP相关分子机制研究的多元化需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Amylin ELISA Kit; DAP ELISA Kit; Diabetes associated peptide ELISA Kit; Diabetes-associated peptide ELISA Kit; IAP ELISA Kit; IAPP ELISA Kit; IAPP_HUMAN ELISA Kit; Insulinoma amyloid peptide ELISA Kit; Islet amyloid polypeptide (diabetes associated peptide, amylin) ELISA Kit; Islet amyloid polypeptide ELISA Kit
缩写：IAPP
Uniprot No.：P10997
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays):CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IAPP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 83 | |||||
| Range % | 80-86 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 90-96 | ||||||
| 1:8 | Average % | 105 | |||||
| Range % | 101-109 | ||||||
回收率：
| The recovery of human IAPP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-93 | |||||
| EDTA plasma (n=4) | 102 | 98-106 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected30001.9011.9321.9171.77415001.5471.5271.5371.3947501.1251.1361.1310.9883750.7980.7780.7880.645187.50.5370.5280.5330.390940.3260.3120.3190.176470.2440.2410.2430.10000.1450.1400.143 | |||||||
货期：3-5 working days
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