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产品描述：人αL艾杜糖苷酸酶(IDUA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物、组织匀浆样本中的IDUA含量。IDUA是α-L-艾杜糖醛酸酶的编码基因，该酶参与糖胺聚糖降解。其缺乏会引发黏多糖贮积症I型，导致多系统病变和发育异常。目前研究聚焦于通过基因治疗、酶替代治疗等机制来补充IDUA功能，改善患者症状和预后。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研领域中对 IDUA 表达水平的分析，例如疾病机制研究、基因治疗模型评估或药物干预效果验证等；为研究溶酶体功能异常、代谢调控及遗传性疾病相关通路提供了可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IDUA ELISA Kit; Alpha-L-iduronidase ELISA Kit; EC 3.2.1.76 ELISA Kit
缩写：IDUA
Uniprot No.：P35475
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell lysates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IDUA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 96-108 | |||||
回收率：
| The recovery of human IDUA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 93-96 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.8252.8672.8462.73952.1032.1192.1112.0042.51.1251.1371.1311.0241.250.5880.5640.5760.4690.6250.3780.3560.3670.2600.3120.2350.2520.2440.1370.1560.1910.1820.1870.08000.1090.1050.107 | ||||||
货期：3-5 working days
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