首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠β干扰素(IFN-β/IFNB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNB1含量。IFNB1即干扰素β1，它是一种细胞因子，在免疫调节中发挥关键作用。研究发现它能抑制病毒复制、调节免疫细胞活性。在医学上常用于多发性硬化症等疾病治疗，其机制是通过与受体结合，激活下游信号通路，发挥免疫调节和抗病毒等效应。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本试剂盒可为病毒感染模型构建、免疫调节机制研究及药物干预效果评估提供定量数据支持，尤其适用于动物实验中对IFN-β分泌水平的分析，如中枢神经系统炎症、病毒性肝炎或自身免疫性疾病等研究场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ifnb1 ELISA Kit; IfnbInterferon beta ELISA Kit; IFN-beta ELISA Kit
缩写：IFNB1
Uniprot No.：P70499
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 98-110 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of pig LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 87 | 80-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.5162.5872.5522.3805002.0212.1062.0641.8922501.3621.3241.3431.1711250.7610.7390.7500.57862.50.5030.5340.5190.34731.250.3750.3590.3670.19515.60.2760.2950.2860.11400.1740.1700.172 | ||||||
货期：3-5 working days
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