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产品描述：牛γ干扰素(IFN-γ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNG含量。IFNG即干扰素-γ，是一种具有多种生物学功能的细胞因子。它在免疫调节中发挥关键作用，能激活巨噬细胞、增强抗原呈递，参与固有免疫和适应性免疫。研究聚焦其信号通路及与疾病关联，如自身免疫病、感染性疾病等，以探索相关治疗策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于畜牧兽医研究中评估牛群免疫状态、疫苗免疫效果验证、病原感染后免疫反应监测等科研场景，同时可为反刍动物疾病模型构建提供关键免疫指标数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFNG ELISA Kit; Interferon gamma ELISA Kit; BoIFNG ELISA Kit; IFN-gamma ELISA Kit
缩写：IFNG
Uniprot No.：P07353
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 99-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 93-101 | |||||
回收率：
| The recovery of bovine IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-96 | ||||
| EDTA plasma (n=4) | 95 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20001.9741.8821.9281.785 10001.2901.1961.2431.100 5000.8180.8070.8130.670 2500.5190.5020.5110.368 1250.3240.3330.3290.186 62.50.2680.2800.2740.131 31.250.2120.2210.2170.074 00.1420.1440.143 | ||||||
货期：3-5 working days
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