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产品描述：人干扰素ε(IFNE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNE含量。IFNE（干扰素-ε）是一种免疫调节蛋白，具有抗病毒和抗菌作用。其研究机制涉及调节免疫细胞功能，增强免疫应答。IFNE在生物医学中的应用包括病毒和细菌感染的治疗，以及免疫相关疾病的预防和治疗。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品适用于科研领域对IFNE表达水平的定量分析，例如研究病毒感染模型中IFNE的动态变化、探索炎症性疾病中黏膜免疫调节机制，或评估生殖系统相关疾病中IFNE的潜在生物标志物功能，为免疫学、病毒学及生殖医学研究提供可靠的技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFNE ELISA Kit; IFNE1 ELISA Kit; UNQ360/PRO655 ELISA Kit; Interferon epsilon ELISA Kit; IFN-epsilon ELISA Kit; Interferon epsilon-1 ELISA Kit
缩写：IFNE
Uniprot No.：Q86WN2
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IFNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 85-95 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 88-99 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 88-99 | |||||
回收率：
| The recovery of human IFNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-100 | ||||
| EDTA plasma (n=4) | 93 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.0582.0112.0351.9315001.6641.5471.6061.5022501.1881.1491.1691.0651250.7390.7040.7220.61862.50.5610.5210.5410.43731.20.3750.3540.3650.26115.60.2190.2110.2150.11100.1050.1030.104 | ||||||
货期：3-5 working days
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