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产品描述：鸡γ干扰素(IFN-γ)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNG含量。干扰素-γ（IFNG）是一种II型干扰素，主要由自然杀伤细胞和活化的T细胞产生，具有抗病毒、免疫调节和抗肿瘤特性。其通过结合干扰素伽玛受体（IFNGR）调节JAK-STAT通路，激活抗原提呈细胞，促进I型辅助T细胞（Th1细胞）的分化，并抑制破骨细胞形成。研究机制涉及多种生物过程和细胞信号通路，如蛋白导入细胞核、转录调控、细胞凋亡和免疫反应等。试剂盒检测范围为3.125 pg/mL-800 pg/mL，适用于家禽免疫机制研究、疫苗效力评估以及感染性疾病模型中免疫应答动态监测，如禽流感、新城疫等病毒性疾病的免疫调控机制探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写：IFNG
Uniprot No.：P49708
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：3.125 pg/mL-800 pg/mL
灵敏度：3.125 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of chicken IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-101 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 8000.1780.1820.180 2000.2450.2330.239 500.4570.4820.470 12.50.8480.8170.833 3.1251.3961.3641.380 02.2372.2422.240 | ||||||
货期：3-5 working days
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