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产品描述：猪γ干扰素(IFN-γ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNG含量。IFNG即干扰素-γ，是一类具有多种生物学活性的细胞因子。它在免疫调节、抗病毒和抗肿瘤等方面发挥关键作用。研究机制上，IFNG能激活免疫细胞，增强其杀伤功能，还可调节多种免疫相关基因表达，是免疫领域的重要研究靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于猪类动物模型中感染性疾病研究、疫苗免疫效果评估、免疫调节机制探索等科研领域，为评估宿主免疫状态及炎症反应提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFNGInterferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写：IFNG
Uniprot No.：P17803
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of pig IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 86-102 | ||||
| EDTA plasma (n=4) | 96 | 89-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.6922.6472.6702.581 5002.4042.4652.4352.346 2501.9741.9891.9821.893 1251.5231.5411.5321.443 62.50.9450.9420.9440.855 31.20.6130.6350.6240.535 15.60.3370.3240.3310.242 00.0900.0870.089 | ||||||
货期：3-5 working days
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