首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠γ干扰素(IFN-γ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的IFNG含量。IFNG（干扰素-γ）是一种重要的细胞因子，主要参与调节先天性和适应性免疫网络。它能够激活巨噬细胞和T淋巴细胞，对病毒和肿瘤的免疫监视具有重要作用。研究显示，IFNG通过JAK-STAT信号通路调节免疫反应，在抗病毒和抗肿瘤免疫中发挥关键作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研实验中免疫应答机制研究、疾病模型免疫状态评估或药物干预后细胞因子动态监测等领域，例如病毒感染模型中Th1型免疫反应的定量分析，或肿瘤微环境研究中免疫细胞活性的功能性评价本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ifng ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写：IFNG
Uniprot No.：P01580
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 82-99 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 87-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of mouse IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 99 | 92-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.8892.7852.8372.670 5002.3562.3242.3402.173 2501.5711.6141.5931.426 1250.8850.8620.8740.707 62.50.5690.5420.5560.389 31.20.3150.3300.3230.156 15.60.2540.2470.2510.084 00.1640.1690.167 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse IFN-γ This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
Biotin-labeled IFN-γ antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
Biotin-antibody Diluent 1 x 15 ml ---Dilute the Biotin-antibody solution.
HRP-avidin Diluent 1 x 15 ml ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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