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产品描述：山羊γ干扰素(IFN-γ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNG含量。IFNG即干扰素-γ，是一种具有多种生物学功能的细胞因子。其它在免疫调节中至关重要。研究机制上，可激活巨噬细胞、增强细胞毒性T细胞的活性，还能调节Th1/Th2细胞平衡，在抗病毒、抗肿瘤及免疫性疾病等研究中受关注。试剂盒检测范围为7.8 pg/mL-500 pg/mL，其水平变化常用于评估感染性疾病模型、疫苗效力实验或免疫调节机制研究；适用于体外定量分析山羊外周血、组织样本中IFN - γ的动态表达，为畜牧领域免疫相关研究、传染病防控及疫苗开发提供可靠的检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写：IFNG
Uniprot No.：P79154
种属：Capra hircus (Goat)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 99-107 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 89-94 | |||||
回收率：
| The recovery of goat IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-102 | ||||
| EDTA plasma (n=4) | 91 | 88-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.8332.9982.9162.8182502.2572.3252.2912.1931251.7421.7951.7691.67162.51.1111.1181.1151.01731.20.7540.7840.7690.67115.60.3770.4100.3940.2967.80.2680.2510.2600.16200.1010.0940.098 | ||||||
货期：3-5 working days
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