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产品描述：犬γ干扰素(IFN-γ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNG含量。IFNG即干扰素-γ，是一种具有多种生物学功能的细胞因子。它在免疫调节、抗病毒和抗肿瘤等方面发挥重要作用。其研究机制涉及激活免疫细胞、调控炎症反应、诱导细胞凋亡等，为多种疾病的治疗提供了新的靶点和思路。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于科研场景中犬类免疫相关研究，例如评估感染性疾病模型中的免疫应答强度、监测疫苗免疫效果、探究炎症性疾病机制或分析免疫调节药物的作用效果。为犬类免疫学、兽医学研究及生物制剂开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFNGInterferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写：IFNG
Uniprot No.：P42161
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-104 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-102 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 90-101 | |||||
回收率：
| The recovery of dog IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 93-100 | ||||
| EDTA plasma (n=4) | 96 | 89-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2001.8851.8531.8691.7661001.2261.3081.2671.164500.8910.9240.9080.805250.6360.6860.6610.55812.50.4070.4250.4160.3136.250.3100.3180.3140.2113.120.2480.2520.2500.14700.1020.1040.103 | ||||||
货期：3-5 working days
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