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产品描述：人干扰素κ(IFNK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNK含量。IFNK即干扰素κ，是I型干扰素家族成员。它具有抗病毒、抗肿瘤和免疫调节等潜在作用。其研究机制方面，能与细胞表面受体结合，激活相关信号通路，诱导多种抗病毒蛋白产生，发挥免疫调节活性，抵御病原体入侵与肿瘤发展。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于病毒感染机制、自身免疫疾病模型构建、皮肤屏障功能研究等科研领域，还特别适用于免疫微环境分析、细胞因子网络研究等基础科研场景，能为探索IFNK在炎症性疾病、肿瘤免疫调控及组织特异性免疫应答中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFN kappa ELISA Kit; IFN-kappa ELISA Kit; Ifnk ELISA Kit; IFNK_HUMAN ELISA Kit; Interferon kappa ELISA Kit
缩写：IFNK
Uniprot No.：Q9P0W0
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IFNK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
| 1:2 | Average % | 85 | |||||
| Range % | 80-90 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-103 | ||||||
回收率：
| The recovery of human IFNK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 97-105 | |||||
| EDTA plasma (n=4) | 92 | 88-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 10002.6942.7582.7262.622 5002.3092.1822.2462.142 2501.5151.6011.5581.454 1250.9541.0040.9790.875 62.50.5210.5600.5410.437 31.250.2870.2930.2900.186 15.60.1930.2050.1990.095 00.1000.1070.104 | |||||||
货期：3-5 working days
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