Chicken Interferon α,IFN-α ELISA Kit

大包装询价

货号: IPD-E10071Ch

Price： ￥3000

48T 96T

数量：

大包装询价

产品描述：鸡α干扰素(IFN-α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNA1含量。IFNA1即干扰素α1，是I型干扰素家族成员。它具有抗病毒、抗肿瘤和免疫调节等多种作用。其研究机制主要是通过与细胞表面受体结合，激活下游信号通路，诱导产生多种抗病毒蛋白，抑制病毒复制和调节机体免疫反应。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于科研领域对禽类免疫系统的机制研究；支持科研人员在病毒感染模型构建、疫苗免疫效果评估、免疫调节剂开发等研究中动态监测天然免疫应答强度；为禽类免疫学研究提供标准化工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFNA1; ELISA Kit; IFNA2 ELISA Kit; Interferon type A1/A2 ELISA Kit
缩写：IFN-α
Uniprot No.：P42165
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-95
1:2	Average %	95
	Range %	91-101
1:4	Average %	93
	Range %	86-98
1:8	Average %	95
	Range %	82-98

回收率：

The recovery of chicken IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	96	92-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/mlOD1OD2AverageCorrected2001.7261.7451.7361.6311001.3361.3271.3321.227500.9570.9760.9670.862250.6210.6310.6260.52112.50.4370.4090.4230.3186.250.3030.3010.3020.1973.120.2340.2440.2390.13400.1070.1020.105

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.