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产品描述：小鼠α1干扰素(IFN-α1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Ifna1含量。Ifna1，即干扰素-α1，属于I型干扰素家族，由巨噬细胞产生，具有抗病毒活性。它通过参与JAK1/TYK2通路，抑制病毒复制并调节免疫反应。研究显示，IFNA1在免疫调节和抗肿瘤反应中发挥重要作用。试剂盒检测范围为3.12 pg/mL-200 pg/mL，该产品广泛应用于病毒感染模型构建、免疫调控机制研究、疫苗或药物干预效果评估等体外实验场景，支持科研人员定量分析不同实验条件下IFN-α1的动态表达变化，为免疫相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ifna1 ELISA Kit; Interferon alpha-1 ELISA Kit; IFN-alpha-1 ELISA Kit
缩写：IFN-α1
Uniprot No.：P01572
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-α1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 82-99 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 83-107 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 93-105 | |||||
回收率：
| The recovery of mouse IFN-α1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-95 | ||||
| EDTA plasma (n=4) | 102 | 93-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.4932.4892.4912.3531001.7701.7991.7851.647501.1641.1221.1431.005250.7450.7680.7570.61912.50.3820.3970.3900.2526.250.2730.2930.2830.1453.120.2200.2320.2260.08800.1370.1390.138 | ||||||
货期：3-5 working days
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