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产品描述：犬α-1/2干扰素(IFN-α1/2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IFN-A1/2含量。IFN-A1/2属干扰素家族，在机体免疫防御中起关键作用。研究发现其能激活细胞内信号通路，诱导抗病毒蛋白表达以抵御病毒感染。还参与调节免疫细胞活性和功能，在对抗肿瘤及自身免疫病中展现潜在价值，是研究热点。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于科研场景中犬类I型干扰素的功能研究；其动态水平变化常被用于评估病毒感染模型、疫苗免疫原性实验或免疫调节相关机制研究；细胞培养上清液可分析体外刺激后免疫细胞分泌情况，组织匀浆则适用于疾病模型中局部干扰素水平的定量分析；为动物免疫学、病毒学及炎症机制研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Interferon alpha-1/2 ELISA Kit; IFN-alpha-1/2 ELISA Kit
缩写：IFN-α1/2
Uniprot No.：P81255
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine IFN-α1/2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 86-99 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 94-109 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 89-96 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 83-96 | |||||
回收率：
| The recovery of canine IFN-α1/2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-96 | ||||
| EDTA plasma (n=4) | 94 | 88-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8002.8152.8242.8202.7264002.4172.4252.4212.3272001.8341.8631.8491.7551001.0421.1121.0770.983500.5180.5630.5410.447250.2480.2690.2590.16512.50.1260.1310.1290.03500.0910.0960.094 | ||||||
货期：3-5 working days
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