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产品描述：鸡β干扰素(IFN-β/IFNB)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFN-β/IFNB含量。IFN-β/IFNB即β-干扰素，是机体受病毒感染后产生的一类细胞因子。它能与细胞表面受体结合，激活下游信号通路，诱导抗病毒蛋白产生，抑制病毒复制和调节免疫反应，在抗病毒、抗肿瘤及免疫相关疾病研究中受关注。试剂盒检测范围为2.5 pg/mL-200 pg/mL，适用于科研场景中禽类免疫机制研究、疫苗效力评价或抗病毒药物开发等方向；可应用于评估病毒感染模型、免疫刺激实验或比较不同组织微环境中IFN-β的动态表达水平本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFNB ELISA Kit; IFN2 ELISA Kit; Interferon type B ELISA Kit; IFN-beta ELISA Kit
缩写：IFN-β/IFNB
Uniprot No.：Q90873
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：2.5 pg/mL-200 pg/mL
灵敏度：3.125 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 95-103 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 88-98 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 84-96 | |||||
回收率：
| The recovery of chicken IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 88-94 | ||||
| EDTA plasma (n=4) | 88 | 82-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 2000.3420.3410.342 750.4940.4860.490 37.50.6520.6640.658 12.51.0671.0761.072 2.51.4781.5751.527 01.8541.9551.905 | ||||||
货期：3-5 working days
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