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产品描述：猪β干扰素(IFN-β/IFNB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的IFN-β/IFNB含量。IFN-β/IFNB即β-干扰素，它具有抗病毒、免疫调节等作用。在研究机制上，它能与细胞表面受体结合，激活相关信号通路，诱导抗病毒蛋白产生，从而抑制病毒复制。目前在治疗多发性硬化症等疾病方面有重要研究价值。试剂盒检测范围为50 pg/mL-2000 pg/mL，该产品适用于体外科研场景中猪IFN-β的动态水平监测，如病毒感染后免疫应答研究、炎症模型评估或生物制剂开发过程中的效价分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IFN-β/IFNB
Uniprot No.：Q29412
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：50 pg/mL-2000 pg/mL
灵敏度：25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 85-86 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-99 | |||||
回收率：
| The recovery of pig IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-101 | ||||
| EDTA plasma (n=4) | 84 | 80-88 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 20002.8972.8512.874 10002.0892.0722.081 5001.1961.1631.180 2500.6490.6870.668 1000.2860.2930.290 500.1440.1460.145 | ||||||
货期：3-5 working days
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